洼地绵羊FecB基因多态性与其产羔数关系的研究  

Association of Polymorphisms in the FecB Gene with Litter Size in Wadi Sheep

作　　者：任艳玲[1,2] 沈志强[1,2] 李敏 肖娜 董文艳 李金林[1,2] 王建军[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东省洼地绵羊繁育技术研究推广中心,山东滨州256600 [3]山东绿都生物科技有限公司,山东滨州256600

出　　处：《中国畜牧兽医》2011年第7期159-162,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)(2008AA10Z142);山东省自然科学基金(Y2007D12);山东省良种工程(2006GG22)

摘　　要：为了从分子水平上研究洼地绵羊的多羔机制,本研究采用PCR-RFLP方法分析洼地绵羊中FecB基因多态性及其与产羔数的关系。结果表明,洼地绵羊中存在与Booroola羊相同的FecB基因突变,有B/B、B/+、+/+3种基因型,其基因型频率分别为0.29、0.56和0.15;且4乳头个体FecB基因频率高于2乳头个体;3种基因型的平均产羔数分别为2.81±0.42、2.49±0.53和1.96±0.28只。经统计分析,B/B与B/+基因型洼地绵羊的产羔数显著高于+/+基因型(P<0.05),B/B与B/+基因型之间差异不显著(P>0.05),而+/+基因型个体也存在多羔现象。结果提示,FecB基因可能是洼地绵羊多羔性能主效基因之一,但洼地绵羊可能还存在其它影响多羔性能的基因。To study the fecundity of Wadi sheep on molecular level,polymorphisms within the FecB gene of Wadi sheep and associations with litter size were analyzed by PCR-RFLP.The results showed that there was FecB mutation in Wadi sheep that was the same as in Booroola,and the frequencies of three genotypes（B/B,B/＋,＋/＋） were 0.29,0.56 and 0.15,respectively.An association between genotype frequencies and number of nipples was observed.Average litter sizes for the three genotypes were 2.81±0.42,2.49±0.53 and 1.96±0.28,respectively.Statistical analysis showed that litter size of Wadi sheep with B/B and B/＋ genotypes were significantly greater than that of sheep with the ＋/＋ genotypes（P〈0.05）,but differences between the B/B and B/＋ genotypes were not significant（P〉0.05）.These results indicated that the FecB gene could be of great interest in the Wadi sheep breed due to its association with fecundity.

关 键 词：洼地绵羊 FECB基因 多态性 产羔数 

分 类 号：S826[农业科学—畜牧学]