间接血凝方法检测副猪嗜血杆菌抗体  

Indirect Haemagglutination Test for Detecting Haemophilus parasuis Antibody

作　　者：徐引弟[1] 王治方[1] 朱文豪[1] 梁跃[1] 许保疆 郭成留[1] 

机构地区：[1]河南省农业科学院畜牧兽医研究所,河南郑州450002

出　　处：《中国畜牧兽医》2011年第7期189-192,共4页China Animal Husbandry & Veterinary Medicine

基　　金：河南省科技攻关项目(072102140004);河南省农科院孵化项目(200712)

摘　　要：将副猪嗜血杆菌（Hps）4、5、13型分离菌株超声产物致敏经戊二醛和鞣酸处理的绵羊红细胞,建立了检测Hps抗体的间接血凝试验方法。对15种血清型Hps阳性血清进行检测,结果均为阳性,抗体效价达1∶25-1∶210,敏感性明显高于琼脂扩散试验。对其它16种病毒和细菌的阳性血清进行检测,结果除胸膜肺炎放线杆菌有凝集外,其余均为阴性。对620份临床血清进行检测,阳性率为67.58%。结果表明,该方法敏感性较高、特异性强,可用于Hps抗体水平的检测和流行病学调查。Indirect hemagglutination assay（IHA） was developped by sensitizing glutaraldehyde-tannin fixed sheep red blood cells with sonicated product of Haemophilus parasuis（Hps） serovar 4,5 and 13 isolated strains.All 15 standard sera of Hps were positive.Antibody titers were 1∶25 to 1∶210,and sensitivity significantly higher than gar gel precipitation test（AGPT）.the positive sera of other 16 viruses and bacterium were negative excluded Actinobacillus pleuropneumoniae（APP）.620 clinical serum were detected and the positive ratio was 67.58%.The results showed that this IHA was sensitive,specific and could be used for antibodies detection and epidemiological investigation of Hps.

关 键 词：副猪嗜血杆菌 间接血凝试验 抗体检测 

分 类 号：S858.28[农业科学—临床兽医学]