γ-干扰素对金黄色葡萄球菌性临床型乳房炎的疗效研究  

Study on Treatment Infect of Interferon-γ to the Clinical Mastitis Induced by Staphylococcus aureus Infection

作　　者：马定坤[1] 伍杰[1] 刘娟[1] 程跃红 

机构地区：[1]阿坝州中等职业技术学校,四川茂县623200 [2]卧龙镇农业服务中心,四川汶川623006

出　　处：《中国畜牧兽医》2011年第7期206-209,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验选取32只3-4 kg健康泌乳家兔,通过人工造模的方法诱发其乳腺发炎,建立金黄色葡萄球菌性临床型乳腺炎模型。然后随机分为A（γ-干扰素）、B（乳酸环丙沙星）、C（γ-干扰素和乳酸环丙沙星）、D（生理盐水）4个治疗组,每组8只,于造模后6、18 h各投药一次。各组分别在造模前1 h和造模后4、12、24、36、487、2 h采集血液0.5 mL、血清1 mL,进行白细胞总数、血清钙离子含量和炎性细胞因子测定（ELISA）,研究其治疗效果。结果显示：①外周血白细胞总数组间对比,A、B组在造模后12-48 h内极显著高于C组（P〈0.01）,72 h时显著高于C组（P〈0.05）,但两组间无显著差异（P〉0.05）;D组在造模后12-72 h内均极显著高于其他3组（P〈0.01）。②血清钙离子含量组间对比,A、B组在造模后12-48 h内极显著低于C组（P〈0.01）,72 h时显著低于C组（P〈0.05）,但两组间差异不显著（P〉0.05）;D组在造模后12-72 h内均极显著低于其他3组（P〈0.01）。③血清中TNF-α和IL-6含量组间对比,A、B组在与C组造模后12-24 h内差异显著（P〈0.05）,36-48 h内差异极显著（P〈0.01）,72 h后差异显著（P〈0.05）,A、B两组间无显著差异（P〉0.05）;D组在造模后12-72 h内均极显著高于其他3组（P〈0.01）。结果表明,γ-干扰素配合乳酸环丙沙星治疗金黄色葡萄球菌性临床型乳房炎具有一定的协同作用。In the experiment,104 CFU/mL/3 to 4 kg（body weight） of Staphylococcus aureus that was isolated from the milk of dairy cattle with mastitis were infused into the third of mammary gland in 32 healthy rabbits,then the clinical mastitis model were challenged.32 rabbits were randomly divided into A（IFN-γ）,B（Ciprofloxacin）,C（IFN-γ and Ciprofloxacin Lactate）,D（normal saline） as four treatment groups,each contains 8 rabbits,then delivery drug only after 6,18 h as first dosage.Each group,respectively,from before established model 1,4,12,24,36,48,72 h observe their temperature,heart rate and respiratory rate and other clinical indicators,and collect blood 0.5 mL,serum 1 mL,for blood and blood biochemical detection,and determination of inflammatory cytokines（ELISA）,to study its therapeutic effect.The results showed that： ① 12 to 48 h after challenged,A,B group,the total number of peripheral white blood cells was significantly higher than the C groups（P〈0.01）,and was higher than the C group（P〈0.05） when the 72 h after challenged,but inter-group no significant difference（P〉0.05）;12 to 72 h after challenged,D group was significantly higher than the A,B,C group（P〈0.01）.Results showed that γ-interferon and the combination of ciprofloxacin in the body increased neutrophil phagocytic leukocytes and bactericidal power is superior to other groups.②12 to 48 h after challenged,A,B serum calcium content was significantly lower than the C group（P〈0.01）,and was lower than the C group（P〈0.05） when the 72 h after challenged,However,inter-group difference was not significant（P〈0.05）;12 to 72 h after challenged,D group was significantly lower than the A,B,C group（P〈0.01）.Showed that the γ-interferon and the combination of ciprofloxacin lactate than alone are more effective in slowing decline in the level of calcium ion concentration,reducing capillary permeability and reduce inflammatory exudation.③C group of TNF-α and IL-6 concentration was lower than A,B g

关 键 词：金黄色葡萄球菌 临床型乳房炎 动物模型 重组γ-干扰素 治疗效果 

分 类 号：S852.61[农业科学—基础兽医学]