中药复方总化学成分(浸膏)提取工艺研究  

The Research of Total Chemical Composition (Extractum) Extracting Technology for Chinese Herbal Compound

作　　者：江平康[1] 刘群[1] 杨松沛[2] 卢亚艺[2] 唐帝[2] 朱子凤[3] 周李[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省兽药监察所,四川成都610041 [3]西南民族大学校医院,四川成都610041

出　　处：《中国畜牧兽医》2011年第7期242-245,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本研究旨在提高白花蛇舌草、金银花、黄芪、甘草组成的中药复方的临床疗效和抗病毒作用机理研究,为复方剂型改革和工业化生产提供科学依据。采用正交设计结合单因素考察,对复方水提和醇沉工艺进行优化:以水提后的浸膏重量为考察指标,选用L9(34)正交表设计试验,对浸泡时间、用水量、煎煮时间、煎煮次数4个因素进行优化;采用HPLC法测定复方浸膏中的甘草酸含量作为醇沉优化指标,选用L9(34)正交表设计试验,对乙醇浓度、乙醇用量、醇沉时间3个因素进行优化。结果得到复方水提最佳工艺:浸泡1 h、12倍加水量、煎煮4次、煎煮时间1.5 h;浸膏醇沉最佳工艺:80%乙醇浓度、4倍乙醇量、醇沉16 h。结合实际生产和成本因素,确定中药复方水提和醇沉工艺为:浸泡0.5 h、12倍加水量、煎煮3次、煎煮时间1 h,65%乙醇浓度、3倍乙醇量、醇沉16 h,水提浸膏收率为57.2%,醇沉浸膏甘草酸含量为0.42%,均接近正交方案中的最高值。The aim of this paper is to improve the clinical curative effect of Chinese herbal compound（Bai hua she she cao,Honeysuckle,Astragalus,Licorice） and promote research of antiviral mechanism of action and provide the scientific basis for chinese herbal compound formulations reform and industrialized production.This study adopted orthogonal design with single factor investigation to optimize compound water extraction and alcohol precipitation technology which were follows： the weight of water extracting was referred as index for checking and choosing L9（34） orthogonal table design test to optimize the time of soaking,water consumption,decoction time,decocting number.The HPLC was used to measure content of glycyrrhizic acid of of chinese compound condensate which was referred as index for optimizing.The L9（34） orthogonal table was selected to optimize ethanol concentration,ethanol dosage and the time of alcohol precipitation.The best technology of water extraction was soaking for 1 hour,adding 12 times alcohol into Chinese medical materials,decocting 4 times and 1.5 hour each.The best technology of the alcohol precipitation was to adding 4 times 80% alcohol into extractum and then sedimenting 16 hours.With the practical production and cost factors,the technology of water extracting and alcohol sedimentating of chinese herbal compound water extracting and alcohol sedimentating was made by the way of soaking one hour,adding 12 times alcohol into chinese medical materials,decocting 3 times and 1 hour each time,adding 3 times 65% alcohol into extractum and then alcohol sedimenting 16 hours.The yield of water extraction extractum was 57.2% and the content of glycyrrhizic acid of extractum was 0.42% which all close to the maximum result in the results of orthogonal design.

关 键 词：中药复方 正交设计 水提工艺 醇沉工艺 

分 类 号：S858.31[农业科学—临床兽医学]