耐碳青霉烯类抗菌药物病原菌的表型检测分析  

Phenotypes analysis of pathogens resistant to carbapenems antibiotics

作　　者：邹桂玲[1] 杜雪飞[1] 孙哲[1] 孟晓玉[1] 陈国玉[1] 

机构地区：[1]哈尔滨医科大学附属第四医院检验科,黑龙江哈尔滨150001

出　　处：《中华医院感染学杂志》2011年第14期3042-3043,共2页Chinese Journal of Nosocomiology

摘　　要：目的了解耐碳青霉烯类抗菌药物病原菌的耐药机制,更合理地指导临床应用碳青霉烯类抗菌药物,降低多药耐药率。方法采用改良Hodge试验方法,同时采用双纸片法进行ESBLs酶检测。结果 145株多药耐药菌中44株(不动杆菌属40株,克雷伯菌属4株)产KPC型碳青霉烯酶。结论产KPC型碳青霉烯酶克雷伯菌属同时产ESBLs酶,耐碳青霉烯类抗菌药物不动杆菌属产KPC型检出率较高。OBJECTIVE To understand the mechanism of drug resistance in carbopenems resistance pathogen, to provide more reasonable guidance for the use of carbapenems antibiotics for clinic,and reduce the rate of multidrug efflus. METHODS ESBLs enzyme was tested by Modified Hodge test and Double-paper method test. RESULTS 44 strains （40 strains Acinetobacter, 4 strains Klibsiella） with KPC producing carbopenems enzyme were detected out for 145 multidrug efflus strains. CONCLUSION Klibsiella producing KPC type of carbopenems enzyme can also produce ESBLs enzyme, the detection rate of KPC produced by Acinetobacter resistant to carbopenems antibiotics is relatively high.

关 键 词：碳青霉烯酶 耐药率 超广谱Β-内酰胺酶 改良HODGE试验 

分 类 号：R378[医药卫生—病原生物学]