心肌细胞特异性Let-7b转基因小鼠的建立  

Establishment of cardiomyocyte-specific Let-7b transgenic mice

作　　者：曹阳[1] 程萱[1] 王剑[1] 范雄伟[2] 杨晓[1] 

机构地区：[1]军事医学科学院生物工程研究所发育与疾病遗传学研究室,北京100071 [2]湖南师范大学生命科学学院心脏发育中心,长沙410081

出　　处：《军事医学》2011年第6期420-423,共4页Military Medical Sciences

基　　金："重大新药创制"科技重大专项资助项目(2009ZX09501-027);国家自然科学基金重点项目(2011CB504200);国家自然科学基金项目(31030040;30700424;31071992)

摘　　要：目的建立心肌细胞中特异性过表达Let-7b的转基因小鼠。方法构建心肌细胞特异性过表达Let-7b的转基因载体,显微注射导入小鼠受精卵中,通过胚胎移植,获得转基因首建者小鼠。利用PCR检测子代小鼠Let-7b基因整合情况。通过实时定量PCR检测Let-7b过表达的效率。结果 PCR检测发现,1只小鼠在其基因组上整合有Let-7b基因。实时定量PCR结果显示,该转基因小鼠在心脏组织中特异性地过表达Let-7b基因。结论成功获得了在小鼠心肌细胞中特异性过表达Let-7b的转基因小鼠。ObjectiveTo obtain transgenic mice that specifically over-express Let-7b in cardiomyocytes.MethodsA construct that directed the over-expression of Let-7b to cardiomyocytes was made and a transgenic mouse was obtained through fertilized egg microinjection followed by embryo transplantation.The integration of Let-7b gene was detected by PCR while the heart-specific over-expression of Let-7b was confirmed by real-time PCR.ResultsOne mouse carrying the Let-7b transgene was identified by PCR.The results of real-time PCR showed that Let-7b was over-expressed in the cardiac tissue.ConclusionCardiomyocyte-specific Let-7b transgenic mice are generated successfully.

关 键 词：Let-7b 肌细胞 心脏 小鼠 转基因 心血管疾病 

分 类 号：R541[医药卫生—心血管疾病] R-332[医药卫生—内科学]