落叶松杂种与亲本ISSR鉴别技术  

ISSR Identification Technology of Hybrid Larch Progenies and Their Parents

作　　者：张含国[1] 张磊[1] 朱航勇 徐悦丽[1] 姚宇[1] 

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040 [2]七台河市园林管理局

出　　处：《东北林业大学学报》2011年第7期1-4,共4页Journal of Northeast Forestry University

基　　金：国家科技基础性工作专项(2007FY110400-3);黑龙江省科技攻关项目(GA09B204-1)

摘　　要：以兴安落叶松(Larix gmelini)、长白落叶松(Larix olgensis)和日本落叶松(Larix Kaempferi)及其杂种子代当年生嫩叶为研究材料,采用ISSR分子标记技术对落叶松亲本与杂种进行了鉴别。结果表明:用ISSR16和IS-SR19号引物扩增的特异条带能够分别鉴别兴安落叶松×长白落叶松杂交种和日本落叶松×长白落叶松杂交种;亲本之一相同的家系间,在已知其相同亲本时,通过特异条带可以计算出另一亲本的可能性;正反交家系间能够扩增出差异率在80%以上的目的条带。在0.4的遗传距离上可以将参试家系划分成4类,即:日本落叶松×兴安落叶松、日本落叶松×长白落叶松、兴安落叶松×长白落叶松和兴安落叶松×日本落叶松4种杂交组合,与分子标记鉴别结果相互印证。A study was conducted to identify hybrid larch progenies and their parents by ISSR markers using the early needles of Larix gmelini, L. olgensis, L. Kaempferi and their hybrid progenies as research materials. Result showed that the hybrids of L. gmelini x L. olgensis and L. kaempferi ×L. olgensis could be identified with specific bands amplified by primer IS- SR16 and ISSR19 respectively. When one parent was the same among different families, the possibility of the other parent could be calculated by amplified bands. Reciprocal cross families could be identified in terms of the appearance rate of target bands more than 80 percent. All the families could be classified into four kinds of hybrids at a genetic distance of 0.4, namely L. kaempferi × L. gmelini, L. kaempferi × L. olgensis, L. gmelini × L. olgensis and L. gmelini × L. kaempferi, which was consistent with the result of molecular markers.

关 键 词：落叶松 杂交种 亲本 ISSR 鉴别 

分 类 号：S722.3[农业科学—林木遗传育种]