西红花酸对大鼠脑缺血-再灌注氧自由基及一氧化氮的影响  

Effect of Crocetin on Free Radicals and Nitrogen Monoxidum During Reperfusion after Cerebral Ischemia in Rats

作　　者：谭安雄[1] 朱耀斌 王玉银[3] 

机构地区：[1]邵阳医学高等专科学校药学系,湖南邵阳422000 [2]湖南交通医院药剂科,长沙410006 [3]中南大学湘雅二医院,长沙410011

出　　处：《医药导报》2011年第7期846-848,共3页Herald of Medicine

基　　金：邵阳市科技计划项目(基金编号:N0962)

摘　　要：目的探讨西红花酸对局灶性脑缺血-再灌注损伤的保护作用及其机制。方法 120只SD大鼠随机分为6组,假手术组、模型组、阳性对照组(给予尼莫地平溶液5 mg.kg-1)及低、中、高剂量药物组(给予西红花酸10,20,40 mg.kg-1),每组20只。假手术组、模型组给予等容量0.9%氯化钠溶液。5组大鼠每日上午灌胃给药1次,连续给药7 d。采用阻塞大脑中动脉制备局灶性脑缺血2 h再灌注22 h模型。观察大鼠神经功能缺陷评分、脑梗死体积,测定缺血脑组织丙二醛(malonaldehyde,MDA)、一氧化氮(nitrogen monoxidum,NO)含量及超氧化物歧化酶(superoxidedismutase,SOD)活性。结果西红花酸高、中、低剂量药物组,尼莫地平组,假手术组,模型组神经功能缺损评分分别为(0.35±0.31),(1.21±0.54),(2.04±0.32),(1.29±0.42),(0.00±0.00),(2.12±0.64)分,脑梗死体积比例分别为(13.2±4.3)%,(21.6±3.5)%,(34.2±2.7)%,(20.6±4.2)%,(0.0±0.0)%,(44.8±3.2)%;MDA分别为(2.0±0.5),(2.9±0.4),(3.8±0.7),(2.5±0.8),(2.1±0.6),(4.2±0.7)μmol.g-1;NO分别为(5.8±1.5),(8.8±1.3),(10.8±2.3),(8.8±2.1),(6.3±1.1),(12.8±1.3)μmol.g-1;SOD分别为(198.3±13.1),(159.8±10.9),(132.4±12.1),(164.3±10.7),(202.6±12.5),(121.1±11.6)kU.g-1。表明西红花酸呈剂量依赖性地降低脑组织梗死体积,改善神经功能,减少脑组织MDA和NO含量,增加脑组织SOD活性。结论西红花酸能保护脑缺血-再灌注损伤,其机制可能与降低脑组织MDA和NO含量、增加脑组织SOD活性有关。Objective To investigate the protective effect and mechanism of crocetin on focal cerebral ischemia-reperfusion injury. Methods 120 SD rats were divided into six groups,named as sham operation（sham group）,model control of cerebral ischemia-reperfusion injury（model group）,nimodipine positive control（Nim group）,high dose of crocetin group（40 mg·kg-1）,moderate dose of crocetin group（20 mg·kg-1）,low dose of crocetin group（10 mg·kg-1）.The sham group and model group were given with the same dose of normal saline.The rats were treated once in each group in the morning by reperfusion for a week.The model of neurological deficits was made by 2 h of middle cerebral artery occlusion followed with 22 h reperfusion.To observe the nerve functional defect score,cerebral infarction,and check the content of malondialdehyde（MDA）,nitrogen monoxidum oxide（NO） and activity of superoxide dismutase（SOD） in ischemic brain tissues. Results The nerve functional defect scores of high,moderate and low dose of crocetin group,Nim group,sham group and model group were（0.35±0.31）,（1.21±0.54）,（2.04±0.32）,（1.29±0.42）,（0.00±0.00）,（2.12±0.64）,respectively;volume ratio of cerebral infarction were（13.2±4.3）%,（21.6±3.5）%,（34.2±2.7）%,（20.6±4.2）%,（0.0±0.0）%,（44.8±3.2）%,respectively;the content of MDA were（2.0±0.5）,（2.9±0.4）,（3.8±0.7）,（2.5±0.8）,（2.1±0.6）,（4.2±0.7） μmol·g-1,respectively;the content of NO were（5.8±1.5）,（8.8±1.3）,（10.8±2.3）,（8.8±2.1）,（6.3±1.1）,（12.8±1.3） μmol·g-1,respectively;the activity of SOD were（198.3±13.1）,（159.8±10.9）,（132.4±12.1）,（164.3±10.7）,（202.6±12.5）,（121.1±11.6） kU·g-1,respectively.Crocetin improved cerebral infarction and nerve function,enhanced SOD activity,reduced MDA and NO content in a dose-dependent manner. Conclusion Crocetin can protect focal cerebral ischemia-reperfusion injury,the mechanism of which may be related to SOD activ

关 键 词：西红花酸 脑缺血-再灌注 局灶性 自由基 一氧化氮 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]