哥斯达黎加链霉菌抗菌成分的活性跟踪分离与鉴定  

Bioassay-guided isolation and identification of antibacterial component of Stretomyces costaricanus

作　　者：郁蕾[1,2] 戴好富[2] 魏景[1,2] 梅文莉[2] 曾会才[2] 

机构地区：[1]海南大学环境与植物保护学院,儋州571737 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《中国抗生素杂志》2011年第7期507-510,共4页Chinese Journal of Antibiotics

基　　金：国家973计划前期研究专项(2009CB125904)

摘　　要：目的分离、鉴定哥斯达黎加链霉菌(Stretomyces costaricanus)发酵液中的抗菌活性成分。方法通过活性跟踪,采用硅胶柱层析等方法对哥斯达黎加链霉菌次生代谢产物进行分离纯化。采用光谱分析对活性成分进行结构解析。结果哥斯达黎加链霉菌中的抗菌活性成分为放线菌素D。结论首次发现放线菌素D对香蕉枯萎镰刀菌4号生理小种(Fusarium oxysporum f.sp.cubense race 4,Focr4),金黄色葡萄球菌(Staphyloccocus aureus)和耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)有较强的抑制活性。Objective To isolate and identify antibacterial component from the fermentation broth of Stretomyces costaricanus. Methods Bioassay-guided silica gel column chromatography were used to separate the antibacterial component from the fermentation broth of Stretomyces costaricanus. The structure of the active component was elucidated by spectroscopic analyses. Results The antibacterial component of Stretomyces costaricanus was actinomycin D. Conclusion It＇s the first report that actinomycin D has strong inhibitory activity against Fusarium oxysporum f.sp. cubense race 4, Focr4, Staphyloccocus aureus and MRSA.

关 键 词：哥斯达黎加链霉菌 放线菌素D 抗菌活性 香蕉枯萎镰刀菌4号生理小种 

分 类 号：Q939.92[生物学—微生物学]