秀丽隐杆线虫-耐药铜绿假单胞菌感染模型的建立  

Establishment of an infection model using Caenorhabditis elegans-multidrug resistance P. aeruginosa

作　　者：周雨朦[1] 陈代杰[1] 李继安[1] 邵雷[1] 朱春宝[1] 

机构地区：[1]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国抗生素杂志》2011年第7期511-514,I0005,共5页Chinese Journal of Antibiotics

基　　金：国家"重大新药创制"科技重大专项资助项目(编号:2009ZX09301-007)

摘　　要：目的建立耐药铜绿假单胞菌感染的秀丽隐杆线虫感染模型。方法利用临床分离的P.aeruginosa A258制备BHI、PGS、NG和Agar线虫感染平板。采用不同浓度的四环素、利福平和多粘菌素B对感染线虫进行治疗,考察感染线虫的存活率。结果不同感染平板对线虫致死情况不同。采用NG感染平板对glp-4;sek-1线虫进行感染,其LT50为3.5d。32μg/mL利福平和16μg/mL多粘菌素B对该感染线虫进行治疗,与不加入药物的对照组相比,其存活率分别提高了5和14倍。但一定浓度的四环素没有提高感染线虫的存活率,在线虫体内不能抑制P.aeruginosa A258的感染,与体外抑菌实验结果相一致。结论建立了秀丽隐杆线虫-耐药铜绿假单胞菌A258感染模型,为此模型用于抗感染化合物的筛选奠定基础。Objective Establishment of an infection model using Caenorhabditis elegans-multidrug resistance P aeruginosa system. Method P aeruginosa A258 strain isolated from clinical patient was used to prepare BHI, PGS, NG and Agar plates for infecting Caenorhabditis elegans. The infected worms were then treated by tetracycline, rifampicin and polymyxin B at different concentrations to investigate the survival rates of infected worms. Results Different infective plates led to different patterns on the worm death. The LT50 was 3.5 days, when glp-4;sek-1 mutant worms were transferred to NG medium. Comparing to control groups without antibiotic, the survival rates of infected worms which were treated with 32μg/mL rifampicin and 16μg/mL polymyxin B were increased to 5 and 14 folds. However, tetracycline which was not active against P aeruginosa A258 in vitro, and did not promote survival of infected worms. Conclusion We had estabilshed the infection model of C. elegans-multidrug resistance P. aeruginosa A258, and the model may build the basic of screening anti-infective compounds.

关 键 词：秀丽隐杆线虫 耐药铜绿假单胞菌 感染模型 

分 类 号：R965.1[医药卫生—药理学]