生长因子对人髁突软骨细胞DNA及胶原合成的影响  

Effects of Growth Factors on DNA and Collagen Synthesis of\;Human Mandibular Condylar Cartilage Cells

作　　者：焦岩涛[1] 王大章[1] 韩文利 李唐新[1] 

机构地区：[1]华西医科大学口腔医学院口腔颌面外科学教研室,610041

出　　处：《华西口腔医学杂志》1999年第4期358-360,共3页West China Journal of Stomatology

基　　金：国家自然科学基金!资助项目 (编号 3 95 0 0 16 4 )

摘　　要：目的:研究胰岛素样生长因子(IGF-)、碱性成纤维样生长因子(bFGF)及转化生长因子-β1(TGF-β1)对人髁突软骨细胞增殖及代谢的调节作用。方法:采用体外细胞培养技术及同位素掺入法。结果:在0.4%新生小牛血清(NCS)条件下,bFGF对人髁突软骨细胞DNA合成有显著的促进作用,浓度在1ng/ml以上具显著性(P<0.05)。IGF-在10～100ng/ml呈剂量效应地促进3H-TdR的掺入,而TGF-β1无显著作用。bFGF在0.1～100ng/ml可显著促进人髁突软骨细胞3H-Proline掺入,最大效应剂量为10ng/ml(增加60%)。IGF-在10～100ng/ml能够明显促进细胞胶原合成,最大值在100ng/ml(增加98%)。TGF-β1在1～10ng/ml明显抑制3H-Proline掺入,最大抑制浓度为1ng/ml,抑制率约为24%。结论:生长因子可有效促进软骨细胞增殖及基质合成。Objective:To investigate the effects of transforming growth factor β\-1(TGF β\-1),insulin like growth factor Ⅰ(IGF Ⅰ)and basic fibroblast growth factor(bFGF)on DNA and collagen synthesis of mandibular condylar cartilage(MCC) of human fetus.Methods: Cell culture, \+3H TdR and \+3H Proline incorporation methods were used.MCC cells were harvested from 4 to 5 months old human fetus. Cells were seeded at 2×10\+4/well on 96 well Plate. After synchronization,medium was replaced by DMEM containing 0.4% NCS with various growth factors and concentrations. Results: bFGF stimulated the DNA synthesis significantly, and IGF Ⅰ had less effect, while the effect of TGF β\-1 was insignificant. For collagen synthesis,bFGF caused a dose dependent increase(60%). A greater effect(98%)was achieved when IGF Ⅰ was added. In contrast, TGF β\-1 could inhibit collagen synthesis(24%). Conclusion: Growth factors play an important part in the proliferation and matrix synthesis of MCC cells,which might be of potential application in treating cartilage destructive lesions.\;

关 键 词：生长因子 髁状突 软骨细胞 胶原合成 DNA 

分 类 号：R681.302[医药卫生—骨科学]