冷胁迫下不结球白菜BcSLAC1基因克隆和表达分析及ABA和H_2O_2在调控气孔运动中的作用  

Cloning,expression of BcSLAC1 induced by cold stress in Brassica campestris ssp.chinensis and the role in regulating stomatal movements of ABA and H_2O_2

作　　者：陈海旺[1] 侯喜林[1] 李英[1] 王枫[1] 杨学东[1] 张硕[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095

出　　处：《南京农业大学学报》2011年第4期8-12,共5页Journal of Nanjing Agricultural University

基　　金：江苏省自然科学基金项目(BK2009311);江苏省农业科技自主创新资金项目(CX(09)604)

摘　　要：采用RT-PCR方法首次获得不结球白菜BcSLAC1基因,该基因核甘酸序列全长1 668 bp,其开放阅读框编码555个氨基酸。与已公布的拟南芥SLAC1基因有较高的同源性。系统进化树分析表明:与SLAHs基因家族其他基因相比,BcSLAC1基因与拟南芥SLAC1基因有更近的遗传距离。在冷胁迫条件下利用气孔开张度测量和荧光显微技术分析表明:冷胁迫下H2O2可能作为下游信号分子参与了保卫细胞中ABA信号转导。实时定量RT-PCR检测表明,冷胁迫下BcSLAC1基因表达量升高,同时用ABA处理后,BcSLAC1基因表达量比抗坏血酸处理后明显上升,说明ABA对BcSLAC1基因的诱导作用和ABA诱导产生的H2O2有关。BcSLAC1 gene was firstly amplified from non-heading Chinese cabbage by RT-PCR method.The nucleotide sequence length of its open reading frame（ORF）was 1 668 bp and encoded 555 amino acid residues,and it showed high homology to Arabidopsis thaliana SLAC1 gene.Phylogenetic tree analysis demonstrated that BcSLAC1 gene had a more recent genetic distance with Arabidopsis thaliana SLAC1 gene compared to the others of SLAHs family.In the cold stress condition,stomatcal aperture bioassay and fluorescence microscopy technology revealed that H2O2 involved as a downstream sigaling molecule of ABA signal transduction to induce stomatal closure in guard cells.Real-time quantitative PCR analysis revealed that the expression of BcSLAC1 gene significantly increased under cold stress and there were significant differences in gene expressions with ABA treatments compared with ascorbic acid treatment.H2O2 was required for the ABA-induced expression of the BcSLAC1 gene in leaves.

关 键 词：不结球白菜 BcSLAC1基因 气孔运动 荧光观察 实时定量PCR 

分 类 号：S634.3[农业科学—蔬菜学]