呼吸窘迫综合征早产儿红细胞天然免疫功能变化  

Changes in erythrocyte natural immunological function in neonates with respiratory distress syndrome

作　　者：范丽莉[1] 刘翠青[1] 孙立新[1] 郑博[1] 焦建成[1] 王焱[1] 夏耀芳[1] 

机构地区：[1]河北省儿童医院新生儿科,石家庄050031

出　　处：《中国新生儿科杂志》2011年第4期229-231,共3页Chinese Journal of Neonatology

基　　金：河北省科学技术研究与发展计划(05276177)

摘　　要：目的探讨呼吸窘迫综合征(NRDS)早产儿红细胞天然免疫功能变化。方法测定24例NRDS早产儿及20例健康早产儿生后24 h及7天时红细胞Ⅰ型补体受体(ECR1)活性及数量。ECR1活性采用红细胞对肿瘤细胞天然免疫粘附功能测定。ECR1分子数量用一步法酶联免疫法测定。采用重复测量的方差分析对两组数据进行比较。结果病例组7天时ECR1数量及粘附活性均高于24 h,差异有统计学意义(P<0.05),病例组ECR1数量及粘附活性在24 h时均低于对照组[数量:(2.11±0.16)比(3.02±0.13),活性:(9.67±1.06)%比(14.70±1.17)%,P均<0.05],7天时两组差异无统计学意义[数量:(2.83±0.12)比(3.13±0.17),活性:(13.46±0.89)%比(14.50±0.98)%,P均>0.05]。两组各时间点ECR1数量与活性均呈正相关(0<r<1,P均<0.05)。结论 NRDS患儿红细胞的天然免疫活性减低。Objective To study the changes of the erythrocyte innate immune function in the patients with neonatal respiratory distress syndrome（RDS）.Methods The expression and activity of CR1 on erythrocytes were measured quantitatively in 24 patients diagnosed with RDS and 20 premature controls without respiratory diseases when they are 24 hours and 7 days old.The innate immune activity of erythrocyte was measured according to erythrocyte quick innate immune adherence reaction on cancer cells.Quantitative expression of CR1 on erythrocytes was measured by one-step enzymelinked immunosorbent assay.The repeated measurement design analysis of variance was used to compare the data between the two groups.Results The quantity and activity of ECR1 of case group in 7 d were significantly higher than that in 24 h（P〈0.05） The quantity and activity of ECR1 were significantly lower in case group in 24 h than in control group.[ECR1 quantity expression（2.11±0.16） vs.（3.02±0.13）,ECR1 activity（9.67±1.06）% vs.（14.70±1.17）%,P〈0.05],There was no statistical significance between the two groups in 7 d [ECR1 quantity expression（2.83±0.12） vs.（3.13±0.17）,ECR1 activity（13.46±0.89）% vs.（14.50±0.98） %,P〉0.05].There is a significant positive correlation between the quantity and activity of ECR1 at any time in both groups（0〈r〈1,P〈0.05）.Conclusion In patients with RDS,erythrocyte innate immune function was found to be low.

关 键 词：呼吸窘迫综合征 新生儿 红细胞 受体 补体 

分 类 号：R722.6[医药卫生—儿科]