利用^(15)N标记研究氮素水平对大豆根瘤生长的影响  

Effects of nitrogen levels on nodule growth of soybean using ^(15)N tracing method

作　　者：董守坤[1] 刘丽君[1] 孙聪姝[1] 张冰[1] 马秀峰[1] 焦光纯[1] 马春梅[1] 龚振平[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030

出　　处：《植物营养与肥料学报》2011年第4期985-988,共4页Journal of Plant Nutrition and Fertilizers

基　　金：黑龙江省科技攻关计划(GA09B103;GA09B104);黑龙江省教育厅科研项目(10531010);东北农业大学博士启动基金资助

摘　　要：为了明确氮肥对大豆根瘤生长的影响,以东农47为试验材料,采用15N标记和砂培的方法,进行了氮素营养水平对大豆根瘤生长及与根瘤固氮相关性的研究。结果表明,随外源氮水平增加,根瘤干重呈先增加后降低的变化趋势,当营养液氮浓度为50 mg/L时,有利于根瘤的生长;根瘤生长需要一定量"启动氮","启动氮"的作用维持到第3片复叶完全展开时(V3)对大豆根瘤的生长最有利;在盛荚期(R4)和鼓粒始期(R5)补充外源氮的供给抑制根瘤的生长。根瘤干重与植株中根瘤氮积累量、根瘤氮比例呈极显著正相关,根瘤干重与植株中氮积累量呈显著正相关。In order to definite the effects of soybean nodules growth,15N tracing and sand culture were used to study the effects of nitrogen nutrition levels on nodule growth and nodule nitrogen fixation of soybean cultivar,Dongnong 47（DN 47）.The results show that the dry weights of nodules are increased earlier and decreased later when the exogenous-N levels are gradually increased,and the dry weight of nodules is maximum when the nitrogen concentration is 50 mg/L.Nodules growth needs ＂startup-N＂,and the best effect of the ＂startup-N＂ on nodule growth is before the third compound leaf expanded（V3） period.Growth of nodules is inhibited while applying exogenous-N from the full pod to beginning seed（R4-R5）.There are significant correlations between dry weight of nodules,N-accumulation of nodules,N ratio in nodules and N-accumulation in plants.

关 键 词：大豆 氮素 根瘤干重 根瘤固氮 

分 类 号：S565.1[农业科学—作物学] S154.381