糖尿病大鼠肺组织超微结构病理变化及罗格列酮干预研究  

Pathological ultrastructure changes of lung tissue in diabetic rat and the preventive effect of rosiglitazone interventon

作　　者：穆维新[1] 沈亚欣[2] 周红[3] 黄敬[3] 王绵[3] 刘光[1] 

机构地区：[1]河北医科大学第四医院东院,石家庄050000 [2]河北医科大学外科总论教研室 [3]河北医科大学第二医院内分泌科

出　　处：《中国综合临床》2011年第7期700-702,共3页Clinical Medicine of China

基　　金：河北省卫生厅医学科学研究重点课题（20100418）

摘　　要：目的观察糖尿病大鼠肺组织的超微结构病理变化及其罗格列酮干预效果。方法通过高糖、高脂饮食加腹腔注射小剂量链脲佐菌素（STZ）的方法，建立2型糖尿病大鼠模型；采用透射电镜观察对照组、单纯糖尿病组、罗格列酮干预组3组大鼠肺组织超微结构改变；同时测定羟脯氨酸含量的变化。结果单纯糖尿病大鼠肺组织出现了胶原纤维的聚集，细胞外基质的增加，出现了肺纤维化，罗格列酮组大鼠肺组织的胶原聚集明显减轻；12周[（0．77±0．03）μg/（mg·prot）与（0．63±0．03）μg/（mg·prot），t=7．071]、20周[（0．93±0．10）μg/（mg·prot）与（0．87±0．10）μg/（mg·prot），t=0．831]单纯糖尿病组肺组织中羟脯氨酸均高于对照组（P均〈0．05）。20周罗格列酮干预组的羟脯氨酸低于同期单纯糖尿病组[（0．85±0．08）μg/（mg·prot）与（0．87±0．10）μg/（mg·prot）t=0．834，P〈0．05）。结论糖尿病大鼠出现了肺纤维化，罗格列酮干预能减轻糖尿病大鼠肺纤维化的程度。Objective To observe the pathological ultrastructure changes of lung tissue in diabetic rat with pulmonary fibrosis and the preventive effect of rosiglitazone intervention. Methods The experimental type 2 diabetic rats were yielded by high-sugar, high fat diet plus intraperitoneal injecting streptozotocin （STZ）. The pathological uhrastructure changes of lung tissue were observed by transmission electron microscopy in the control.diabetes mellitus zroup and rosiglitazone treatment group respectively, and the hydroxyproline contentswere determined simultaneously. Results We observed accumulation of collagen fibers and extracellular matrix in the lung tissue of simple diabetic rat, which indicated pulmonary fibrosis. The hydroxyproline contents were significantly higher in diatetes mellitus group when compared to the control at 12 weeks [ 0. 77 ± 0. 03 μg/（ mg· prot） vs. 0. 63 ± 0. 03 μg/（ mg · prot） ] and 20 weeks [ 0. 93± 0. 10μg/（ mg · prot） vs. O. 87 ± 0. 10 μg/（ rng · prot） ]. （Ps 〈 0. 05 ）, but the rosiglitazone intervention significantly reduced the hydroxyproline contents in diabetes mellitus rats [0.85±0.08 μg/（mg · prot） vs. 0. 87·0.10 μg/（mg · prot） ,P 〈0.05]. Conclusion Pulmonary fibrosis occurred in diabetic rats lung tissue,while rosiglitazone can alleviate it.

关 键 词：糖尿病 大鼠 超微结构 肺纤维化 罗格列酮 

分 类 号：R587.1[医药卫生—内分泌]