四倍体及二倍体毛泡桐截干再生特性研究  

Study on Stumped Regeneration Tetraploid and Diploid Paulownia tomentosa(Thunb.)Steud.

作　　者：梅辉[1,2] 王莉[1] 宋兆建[1] 王维[1] 张献华[1] 陈冬玲[1] 何玉池[1] 蔡得田[1] 

机构地区：[1]湖北大学生命科学学院,武汉430062 [2]武汉生物工程学院生物技术系,武汉430415

出　　处：《湖北林业科技》2011年第3期22-25,54,共5页Hubei Forestry Science and Technology

基　　金：武汉市科技攻关计划项目(20032003031)资助

摘　　要：为掌握人工诱导四倍体毛泡桐保持多倍体特性情况及毛泡桐截干再生特性,笔者以5a生四倍体及二倍体毛泡桐截干再生苗为材料,对截干再生苗的倍性、出芽数、生长速率、叶片特性、叶绿素含量及光合特性等进行研究。结果表明:四倍体毛泡桐截干再生苗能很好地保持其多倍体倍性,相对于二倍体来说,出芽数较少,生长较缓慢;气孔增大,气孔密度降低;叶片叶绿素含量降低,但光合速率增大。叶片形状二者也有很大差异,四倍体为锯齿状卵圆形而二倍体为全缘五边形。另外,嵌合体很少见,显现了组织培养诱导多倍体的优势。而且多倍体毛泡桐节间变短、叶圆厚和花增大的特点很有利于园林观赏乔木花卉的培育。In order to know the ability of keeping polyploidy of artificial induction and the growth habits of Paulownia tomentosa（Thunb.）Steud.after being stumped,the polidy,the sprout number,growth rate,leaf characteristics,chlorophyll contents,and photosynthesis characteristics of stumped regeneration five-year-old tetraploid and diploid Paulownia tomentosa（Thunb.）Steud.were studied by authors.The results showed that the stumped regeneration tetraploid Paulownia tomentosa（Thunb.）Steud.can steadily keep the polyploidy ploidy.The differences between tetraploid and diploid Paulownia tomentosa（Thunb.）Steud.were found as follows lesser sprout number and slower growth rate in tetraploid.The mean length of the stomata was longer in tetraploid plants than diploid plants,and stomatal frequency was reduced with the increased ploidy level.Chlorophyll content in leaves reduced but photosynthetic rate increased in tetraploid.The tetraploid and diploid plant differed significantly in leaf shape,with those of the former being round with serrate margin and those of the latter pentagonal with entire margin.In addition,few chimeras demonstrated the superiority of inducing polyploid by tissue culture.The characters of shorter internodes,thicker leaves and bigger flowers of polyploid Paulownia tomentosa（Thunb.）Steud.were very propitious to breeding garden plants,include arbor and flowers.

关 键 词：毛泡桐 多倍体 生长习性 光合特性 

分 类 号：S511[农业科学—作物学]