宁夏汉族Graves病与CYP27B1基因启动子-1260C/A多态性相关性研究  

Association between CYP27B1 Gene Promoter Polymorphisms and Patients with Graves' disease in Ningxia Han People

作　　者：康毓芝[1] 陈耀平[2] 杨宝珍[2] 王立斌[2] 吴若芬[2] 师志云[2] 尹琳琳[1] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院医学实验中心,银川750004

出　　处：《宁夏医科大学学报》2011年第6期540-542,共3页Journal of Ningxia Medical University

基　　金：宁夏回族自治区研究生教育创新计划项目(D09004)

摘　　要：目的探讨CYP27B1(Cytochromo P450 CYP)基因启动子C-1260A位点多态性与宁夏汉族Graves病(GD)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction restriction frag-ment length polymorphism,PCR-RFLP)技术,分析汉族GD 99例和正常对照83例CYP27B1基因启动子-1260C/A多态性。结果宁夏汉族CYP27B1基因-1260C/A检出GD组与对照组的基因型频率分别为37.37%、53.54%、9.09%和43.37%、40.96%、15.66%,两组差异无统计学意义(χ2=4.19,P>0.05);等位基因频率为64.16%、35.86%和63.86%、43.14%,两组差异无统计学意义(χ2=0.00,P>0.05)。结论 CYP27B1基因1260C/A的与宁夏地区汉族GD无相关性。Objective To investigate the association between CYP27B1 gene promoter polymorphisms and patients with Graves＇ disease in Ningxia Han People.Methods A case control study was performed for CYP27B1（1260 C/A） in the promoter region in 99 patients with GD and 83 healthy individuals.The CYP27B1 genotype all cases and control were examined of by polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）.Results In the promoter（1260） of CYP27B1 gene,the frequencies of genotypes and C/A allele showed no significant difference between cases and controls（P0.05）.Conclusion The position 1260 C/A in the promoter of CYP27B1 may be not susceptible to GD in Ningxia Han people.

关 键 词：GRAVES病 CYP27B1基因 基因多态性 

分 类 号：R581[医药卫生—内分泌]