DPPH·法测定红柳醇提物各组分清除自由基的能力  

Determination of Activities of Extracts from Tamarix Chinensis L. Ability of Scavenging Free Radical by DPPH· Assay

作　　者：黄晓会[1] 解宁湘[1] 姚遥[1] 张振华[1] 王培香[1] 王妍[1] 

机构地区：[1]宁夏医科大学基础医学院化学系,银川750004

出　　处：《宁夏医科大学学报》2011年第6期562-564,共3页Journal of Ningxia Medical University

摘　　要：目的通过11,二苯基-2-苦肼基自由基(DPPH.)法测定红柳醇提物各组分清除自由基的能力。方法将红柳醇提物依次用石油醚、乙酸乙酯、正丁醇进行萃取,各组分都配置成相当于原药材50mg.mL-1的溶液,通过DPPH.法测定各组分的清除自由基的能力,并计算各组分的清除50%自由基时样品浓度(IC50)。结果正丁醇层清除自由基的能力最强I,C50为0.55mg.mL-1,其次是萃后水层(IC50为1.47mg.mL-1),乙酸乙酯层(IC50为3.96mg.mL-1)和石油醚层(IC50为4.78mg.mL-1)。结论红柳醇提物各组分对DPPH.均具有一定清除作用,具有体外抗氧化性,可作为有效的天然自由基清除剂,具有很大的开发利用前景。Objective To evaluate the determination of DPPH· alcohol extracts of tamarisk of each component of the antioxidant activities.Methods Alcohol extracts of tamarisk were extracted successively with petroleum ether,ethyl acetate,butanol.The components were configured to the equivalent of original medicinal 50mg·mL-1 solution.Free radical scavenging ability of components was determined by the DPPH· and each component of the IC50 was calculated.Results Antioxidant activity of butanol layer was the strongest and IC50 was 0.55mg·mL-1,followed by the water layer after extraction（IC50 was 1.47mg·mL-1）,ethyl acetate layer（IC50 to 3.96mg·mL-1） and the petroleum ether layer（IC50 was 4.78mg·mL-1）.Conclusion Alcohol extracst of tamarisk all the components of the DPPH· has a clear role in vitro antioxidant activities.It can be used as effective natural free radical scavenger and has a great prospect for development and utilization.

关 键 词：红柳 DPPH· 自由基 

分 类 号：R284.1[医药卫生—中药学]