牛血清白蛋白与硝羧偶氮反应的光谱法研究  

Study on Mechanism of BSA Binding with Carboxynitroazo

作　　者：胡秋娈[1] 李克安[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《北京大学学报（自然科学版）》1999年第6期850-854,共5页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金!资助项目 ( 2 93 92 60 1)

摘　　要：0　引　言蛋白质的定量是生物化学、临床医学乃至食品检验等最常涉及的内容。所使用的方法多种多样,其中用小分子作为光谱探针测定蛋白质以其简便、灵敏等优点而被广泛应用,如考马斯亮蓝Ｇ250[1]、溴酚蓝[2]、氨基黑10Ｂ[3]等。但是,这类探针反应的作用机理迄今仍不很清楚...In this paper,it is found firstly that BSA can sensitively react with carboxynitroazo,used as a good colouring reagent of some inorganic ions before,in acid medium and at room temperature.The absorption spectra and its reaction mechanism were studied.BSA binds with carboxynitroazo mainly through the static electric force.The maximum combination number of carboxynitroazo binded on BSA was determined,based on principle of molar ratio method.The results were conform to the change of its spectra.The fundamental conditions of the reaction and the possibility of spectrophotometric determination of proteins using carboxynitroazo were discussed.The determined molar absorptivitys were 2 43×10 5?L·mol -1 ·cm -1 for BSA and 4 48×10 5?L·mol -1 ·cm -1 for γ\|G,respectively.

关 键 词：牛 血清 白蛋白 硝羧偶氮 分光光度法 

分 类 号：Q513.403[生物学—生物化学] O657.32[理学—分析化学]