银粉玻璃离子体的细胞毒性研究  

Study on cytotoxicity of glass ionomer silver in vitro

作　　者：吴利民[1] 吴银洲[2] 

机构地区：[1]咸宁学院生物医学工程学院,湖北咸宁437100 [2]咸宁学院五官医学院,湖北咸宁437100

出　　处：《现代中西医结合杂志》2011年第21期2619-2620,2622,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的研究临床常用的银粉玻璃离子体（日本松风）的体外细胞毒性。方法用细胞培养液浸提银粉玻璃离子体的粉剂（powder）,液剂（liqu id）及粉剂＋液剂（Power＋liqu id）各24 h,配置不同浓度的浸提液（0.1~100 g/L）,将小鼠成纤维细胞（L-929）在不同浓度的浸提液中分别培养24 h,通过A lam ar B lue比色法,检测各组分对L-929细胞相对增殖率的影响,评价其细胞毒性。结果 L-929细胞呈现高的细胞增殖率,各浓度浸提液之间均无显著性差异（P均〉0.05）,其细胞毒性为0~1级。结论临床常用的银粉玻璃离子体无明显的细胞毒性,具有较好的生物相容性。Objective It is to observe the cytotoxicity of glass ionomer silver in vitro.Methods Cell culture medium was exposed to the glass ionomer silver（0.1 to 100 g/L） for a period of 24-hour.The L-929 mouse fibroblasts were treated with cell culture medium for 24-hour.The cell viability rate and growth rate were observed and assessed using Alamar Blue assay.Results L-929 mouse fibroblasts showed higher growth rate.No significant difference in different consistencies were found（all P0.05）,the cytotoxicity of the glass ionomer silver was grade 0 to 1.Conclusion The glass ionomer silver has no significant toxic effects to cells,and has better biocompatibility.

关 键 词：银粉玻璃离子体 细胞毒性 AlamarBlue比色法 成纤维细胞L-929 

分 类 号：R965.3[医药卫生—药理学]