水稻白叶枯病菌北方菌株遗传多样性rep-PCR分析  

Rep-PCR Analysis of Genotypic Diversity of Xanthomonas oryzae pv. oryzae Strains from North China

作　　者：阙海勇[1,2,3] 陈华民[2] 吴茂森[2] 何晨阳[2] 

机构地区：[1]江西出入境检验检疫局综合技术中心,江西南昌330002 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193 [3]江西农业大学农学院,江西南昌330045

出　　处：《江西农业大学学报》2011年第3期482-487,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家农业行业科研专项经费项目(nyhyzx07-056);国家科技支撑计划项目(2006BAD08A04)

摘　　要：为了揭示水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)北方菌株的遗传结构组成,利用rep-PCR技术对103个从辽宁、吉林、河北、山东稻区采集的Xoo菌株、9个标准小种(R1-R9)代表菌株和13个水稻细菌性条斑病菌(X.oryzae pv.oryzicola,Xoc)菌株进行了遗传多样性分析。结果表明,ERIC-PCR和BOX-PCR分析均能较好地揭示出测试菌株的遗传多态性,分别获得了84种和90种分子谱型;在相似系数为70%时,ERIC-PCR将所有测试菌株聚类为17个簇群,其中1个为优势簇群;BOX-PCR将测试菌株聚类为10个簇群,其中2个为优势簇群。在相似系数为50%时,Xoo与Xoc测试菌株分别聚类为2个不同的簇群。To reveal the genetic structure of Xanthomonas oryzae pv.oryzae（Xoo） strains in the north rice-growing regions of China,103 strains isolated from the diseased rice leaves collected from Liaoning,Jilin,Hebei and Shandong Provinces,9 representative strains of pathotypes/races（R1-R9） of Xoo and 13 strains of X.oryzae pv.oryzicola（Xoc） were analyzed using rep-PCR technique.Dendrograms were generated from the combination data sets of primers ERIC and BOX by using UPGMA analysis.The results indicated that extensive genetic diversity existed within the population of Xoo strains tested,they showed 84 and 90 haplotypes respectively.All tested strains could be divided into 17 clusters at level of 70% similarity using primer ERIC,and there was one predominant cluster,but 10 clusters using primer BOX and two predominant clusters.All tested strains of Xoo and Xoc could be divided into two different clusters respectively at a level of 50% similarity.

关 键 词：水稻白叶枯病菌 REP-PCR 分子谱型 簇群 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]