鸡NCOA1基因发育性表达模式的研究  

Developmental Expression Patterns of NCOA1 Gene in Chicken

作　　者：赵振华[1] 黎寿丰[1] 黄华云[1] 李春苗[1] 张静[1] 薛龙岗[1] 丁余荣[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225003

出　　处：《江西农业大学学报》2011年第3期564-567,588,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江苏省"333"工程培养资金项目(BRA2010162);现代农业产业技术体系建设专项资金;中国农业科学院博士基金(JQ2009002)

摘　　要：应用实时荧光定量PCR技术,以不同繁殖性能的母系(S3系)、父系(Y、F、D系)为研究对象,研究了鸡NCOA1基因不同发育时期在下丘脑、垂体、卵巢、肝脏和血液中的相对表达量及表达模式。结果表明,NCOA1基因在鸡性腺轴组织中高水平表达,NCOA1基因在组织中的表达量与鸡性腺发育和产蛋性能呈相似的变化规律。随着生长发育的变化NCOA1基因表达量也呈波动性增加,14周龄前其表达量较少,性成熟后NCOA1基因表达量快速增加,28周龄时表达量达到峰值,之后表达量下降。产蛋性能较好的母系鸡NCOA1基因的相对表达量比产蛋性能较差的父系鸡较早地开始增加,并在14周龄后表达量显著高于父系鸡(P<0.05)。本研究初步揭示了鸡生长发育过程中NCOA1基因表达的发育性变化模式和品系差异,为进一步研究NCOA1基因对鸡繁殖性状的影响提供了基础数据。The paper studieds the populations with different reproduction performance including female line（S 3）,male line（F,D,Y） for identification of the relative expression levels and expression patterns of NCOA1 gene changes in hypothalamus,pituitary,ovary,blood and liver in different development periods by using rea1-time quantitative PCR.The results indicated that the NCOA1 gene expression level in gonad tissues was higher than that in other tissues.The mRNA expression regulation of NCOA1 gene was similar to the growth regulation of chicken gonad tissues and egg-laying.NCOA1 gene expression increased along with growth with undulatory property,and there was little expression before 14 weeks.NCOA1 gene expression rapidly increased along with sex maturation,and high expression was found at 28 weeks then decreased.NCOA1 expression level of the female line was earlier than that of the male line and had significantly higher mRNA expression after 14 weeks（P0.05）.NCOA1 genes may be playing an important role in reproductive process.These results suggested that the mRNA level of NCOA1 gene in gonad tissues was different in postnatal developmental stages among lines,which provided some data for further study on the NCOA1 gene for elucidating the molecular mechanism of reproduction traits.

关 键 词：鸡 实时荧光定量PCR NCOA1基因 表达模式 

分 类 号：S831.2[农业科学—畜牧学]