狗牙根居群遗传分化的RAPD扩增片段频率分析  

Genetic Differentiation in Bermudagrass Populations By RAPD Analysis

作　　者：梁月香[1,2] 梁慧敏[1] 夏阳 燕丽萍 刘翠兰 

机构地区：[1]江苏农林职业技术学院农艺系,江苏句容212400 [2]宁夏农林科学院,银川750002 [3]山东省林业科学研究所,济南250014

出　　处：《湖北农业科学》2011年第13期2706-2709,共4页Hubei Agricultural Sciences

基　　金：江苏省高技术研究项目(BG2007317);江苏省"六大人才高峰"项目(07-G-002)

摘　　要：狗牙根(Cynodon dactylon L.Pers.)不同居群由南向北外部性状具有广泛的变异性,为明确其分化的分子基础,以收集到的28份狗牙根为材料,用20个随机引物(10 bp)共扩增出318个RAPD多态性片段,平均每个引物15.9个多态位点,在24个居群中共检测到了46个特异性片段;3种类型有8%的多态性片段明显不同;相似生境或同一地域的居群在一些位点上表现出相似或相同的变化。There existed abundant morphological variation among populations of bermudagrass（Cynodon dactylon L.Pers.） from south to nouth.To reveal the molecular bases of its genetic differentiation,314 RAPD polymorphism fragments were amplified from 28 samples of bermudagrass by using 20 primers（10nt）.The mean number of amplified polymorphisms was 15.7 per primer.46 fragments from 24 populations were specific.8% polymorphic fragments were evidently different among the three types.Similar or identical variations were represented among the populations in the similar habitat on certain loci.

关 键 词：狗牙根(Cynodon dactylon L.Pers.) 遗传分化 RAPD 

分 类 号：S543.9[农业科学—作物学]