DM协同放疗抑制人胃癌细胞增殖的实验研究  

Experimental Study on the Inhibition of DM Accompanied with Radiotherapy for Proliferation of Human Gastric Cancer Cells

作　　者：殷海涛[1,2] 王彩莲[1] 刘宝瑞[3] 

机构地区：[1]东南大学附属中大医院,江苏南京210009 [2]南京中医药大学第一临床医学院,江苏南京210046 [3]南京大学医学院附属鼓楼医院,江苏南京210008

出　　处：《南京中医药大学学报》2011年第4期355-357,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：南京市医学科技发展基金(YKK09097)

摘　　要：目的观察连翘中分离所得三萜类化合物达玛-24-烯-3β-乙酰氧基-20S-醇(Dammar-24-ene-3-βacetate-20S-ol,DM)对人低分化胃癌细胞BGC-823的增殖抑制作用及DM的放疗增敏作用。方法流式细胞仪检测不同浓度DM液对BGC-823细胞周期、凋亡的影响;克隆形成法测定细胞存活分数;计算各项放射学参数D0、Dq、N及放疗增敏比。结果 DM可以诱导BGC-823细胞凋亡,引起细胞周期中G1期比例增高,而S期比例降低。25μg/mL DM液作用BGC-823细胞48 h显示出有放疗增敏作用,放疗增敏比达1.97。结论 DM可以引起BGC-823细胞凋亡,DM有放疗增敏作用。OBJECTIVE To observe the inhibition of Dammar-24-ene-3β-acetate-20S-ol（DM） obtained from Fructus Forsythiae through separation for the proliferation of human poor differentiated gastric cancer cells BGC-823 as well as DM＇s radio therapeutic sensitization.METHODS The effects of DM solutions of different concentrations on BGC-823 cell cycle and apoptosis were detected by flow cytometry.Clonogenic assay was applied for cellular survival fraction.Radiological indexes including D0,Dq,N and enhanced sensitivity rate were calculated.RESULTS DM induced the apoptosis of BGC-823,increased the ratio of G1 period,but reduced the ratio of S period.25μg/mL DM solution acting on BGC-823 for 48h showed enhanced sensitivity after radiotherapy with the rate of 1.97.CONCLUSION DM can induce BGC-823＇s apoptosis and is of radiation-sensitivity enhancing effect.

关 键 词：连翘 达玛-24-烯-3β-乙酰氧基-20S-醇 放射治疗 胃癌 

分 类 号：R285.5[医药卫生—中药学]