液相色谱-质谱联用法测定犬血浆中青蒿素浓度  

Determination of artemisinin in dog plasma by LC-MS/MS

作　　者：李超[1] 王锐利[1] 张丽锋[1] 张淑秋[1] 

机构地区：[1]山西医科大学药学院临床药学教研室,太原030001

出　　处：《中国药物与临床》2011年第7期748-749,共2页Chinese Remedies & Clinics

基　　金：国家自然科学基金(30973589;30572367);山西省回国留学人员科研项目(2009-47);山西省青年科技研究基金(2009021040-1);山西医科大学青年基金(02200704)

摘　　要：目的建立犬血浆中青蒿素(ART)浓度的液相色谱-质谱联用方法。方法以蒿甲醚(AMT)为内标,采用乙腈和10mmoL/L醋酸铵(含0.1%甲酸)溶液(85:15,V/V)为流动相,流速为0.3ml/min,Agilent-C18(50mm×4.6mm,1.8μm)色谱柱为分析柱,采用电喷雾电离源(ESI),多反应监测方式进行正离子检测。结果测定犬血浆中青蒿素浓度的线性范围为2～500ng/ml,定量下限为2ng/ml。日内、日间精密度(RSD)均<7.7%,提取回收率78%～90%。结论本方法适应于犬体内青蒿素药代动力学研究。Objective To establish a liquid chromatography-mass spectrometry（LC-MS） method for the determination of artemisinin in dog plasma.Methods Artemether（AMT） was used as the internal standard.Solution of acetonitrile and ammonium acetate at 10 mmol/L（containing 0.1% formic acid）（85：15,V/V） was used for mobile phase preparation with a flow rate at 0.3 ml/min.An agilent-C18 column（50 mm×4.6 mm,1.8 μm） was used for separation.Electrospray ionization（ESI） and multiple reaction monitoring（MRM） were used for positive ion detection.Results The linear calibration curves were obtained in the concentration range of 2～500 ng/ml,with the lowest limit of quantification at 2 ng/ml.Both inter and intra day precision（RSD） were less than 7.7%.The extraction recovery was between 78% and 90%.Conclusion The method is qualified for artemisinin pharmacokinetics study in dogs.

关 键 词：青蒿素 血浆 色谱法 高压液相 光谱分析 质量 

分 类 号：R96[医药卫生—药理学]