白术挥发油提取方法研究  

Extraction of volatile oils from rhizoma atractylodis macrocephalae

作　　者：阎克里[1] 朱秀卿[2] 赵丽[1] 

机构地区：[1]山西省肿瘤医院药物检测室,太原030013 [2]山西省肿瘤医院中药制剂室,太原030013

出　　处：《中国药物与临床》2011年第7期763-767,共5页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关项目(200833)

摘　　要：目的建立白术挥发油高效液相色谱法(HPLC)定量分析方法。研究白术挥发油提取方法。方法对3种有机溶剂和4种提取方法提取的4个产地挥发油提取率及挥发油中5个定量指标含量进行分析比较。结果乙酸乙酯、正己烷、石油醚浸提挥发油提取率依次为2.76%,2.24%,1.96%,超声法的依次为2.54%,1.98%,1.86%。SPE法挥发油提取率为3.13%,水蒸气蒸馏法的为1.42%。挥发油含量浙江>安徽>湖南>河北。水蒸气蒸馏法与其他3种提取方法相比,苍术酮含量低1倍以上,桉叶醇含量低35倍,榄香烯含量为零,白术内酯Ⅰ、白术内酯Ⅲ含量高3倍以上。结论研究建立的挥发油HPLC法定量分析方法简单、稳定可靠。SPE法为白术挥发油较好的提取方法。在没有SPE设备时,乙酸乙酯浸提法是白术挥发油较合适的提取方法。水蒸气蒸馏法提取白术挥发油并不科学。Objective To establish a quantitative analysis for extraction of volatile oils from Rhizoma Atractylodis Macrocephalae by HPLC method.Methods Quantitative analysis and comparison were conducted for contents and extraction rates of 5 active ingredients in volatile oils from rhizoma atractylodis macrocephalae,purchased from 5 geographical regions,and extracted with three organic solvents and 4 extraction methods.Results The extraction rates were respectively,2.76%,2.24% and 1.96% using ethyl acetate,N-hexane and benzine,2.54%,1.98% and 1.86% using ultrasonic method,3.13% by SPE method,and 1.42% by steam distillation.The highest rate of extraction was achieved with products from Zhejiang,followed in order by Anhui,Hunan and Hebei.Compared with the other three extraction methods,volatile oil obtained with steam distillation was one-fold lower in atractylon,35-fold lower in eudesmol,zero contents in elemene,and 3-fold higher in atractylenolids Ⅰ and Ⅲ.Conclusions The quantitative analysis using HLPC for rhizoma atractylodis macrocephalae volatile oil appears simple,stable and reliable.SPE can be a favorable extraction method of rhizoma atractylodis macrocephalae volatile oil.Where SPE facilities are unavailable,ethylacetate leaching method can be an ideal alternative.Steam distillation appears to be a poor method for extraction of Atractylodes macrocephala Koidz volatile oils.

关 键 词：色谱法 高效液相 白术挥发油 提取法 

分 类 号：R284[医药卫生—中药学]