基因修饰的骨髓间充质干细胞复合脱细胞软骨基质促进疏松骨质修复的实验研究  

Experimental study of the composite ECM gene modified MSC to promote osteoporosis

作　　者：邱烺[1] 张丽蓉[2] 沙玉 

机构地区：[1]广东药学院附属第一医院骨科,广州510080 [2]广东药学院病理生理教研室,广州510006 [3]广东省荣誉军人康复医院,广州510260

出　　处：《疑难病杂志》2011年第7期521-523,共3页Chinese Journal of Difficult and Complicated Cases

摘　　要：目的探讨经基因修饰的骨髓间充质干细胞(MSC)与脱细胞软骨基质(ECM)混悬物对骨质疏松模型大鼠的疗效。方法以去卵巢法将30只3月龄SD大鼠制成骨质疏松模型。实验组18只,将SD大鼠MSC分离培养和siRNA(RNA干扰)修饰,与ECM制成混悬物后注入SD大鼠骨质疏松模型椎体骨质中;对照组12只注入生理盐水。观察2组动物不同时间的椎体骨质X线表现差异和力学性能改变。结果 4～12周时,随着周次的增加,实验组动物X线摄片见椎体骨质圆点状密度增高影扩大增加趋势,力学测试最大载荷、弹性模量和最大应变力均较对照组增强,差异有统计学意义(P<0.05)。结论利用经基因修饰的MSC与ECM混悬物注射对骨质疏松实验动物骨质改善效果良好,为探索骨质疏松症的治疗新途径提供一定依据。Objective To investigate the efficacy of genetically modified bone marrow mesenchymal stem cells （MSC） with extracellular cartilage matrix（ECM） material suspension on rat model of osteoporosis.Methods Thirty SD rats （3 months of age） established osteoporosis model by ovariectomized method.MSC of these SD rats were isolated and cultured with siRNA（RNA interference） modified,and ECM made of material suspension was injected into SD rat model of osteoporosis vertebral bone in the experimental group（n = 18）,control group（n = 12） with saline injection.Both the two groups were observed at different times with the X-ray,vertebral bone and mechanical properties change.Results From 4 to 12 weeks, with time increases,vertebral bone density increased with dot-like shadow expand upward in the X-ray can be found in the experimental animals.For the mechanical test maximum load,elastic modulus and maximum strain in the experimental group were increased more obviously than those in the control group,the difference was statistically significant（P0.05）.Conclusion The use of genetically modified MSC and ECM material suspension injection of osteoporosis improved animal bone for the treatment of osteoporosis;and provide information for the treatment of osteoporosis.

关 键 词：骨质疏松症 骨髓间充质干细胞 脱细胞软骨基质 基因修饰 大鼠 

分 类 号：R580[医药卫生—内分泌] R683[医药卫生—内科学]