莱菔硫烷对人肝癌HepG-2细胞ERK信号通路的影响  

Effect of sulforaphane on protein expression of ERK and pathway in human hapetocelluar carcinoma HepG-2 cells

作　　者：邹翔[1,2] 曲中原[2] 白晶[3] 季宇彬[1,2,3] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]哈尔滨商业大学中药学博士后流动站,哈尔滨150076

出　　处：《哈尔滨商业大学学报（自然科学版）》2011年第3期257-258,266,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(30300284);黑龙江省自然科学基金项目(D200802);黑龙江省骨干教师项目(1154G36)

摘　　要：研究莱菔硫烷(sulforaphane,SFN)对ERK、p-ERK蛋白表达的影响,探讨其诱导人肝癌HepG-2细胞凋亡的机制.采用Western Blot方法检测人肝癌HepG-2细胞内ERK及p-ERK蛋白的表达.结果表明,10、20、40μmol/L的SFN作用人肝癌HepG-2细胞48 h可显著下调HepG-2细胞内p-ERK蛋白的表达,而对ERK的表达无明显影响.提示SFN可抑制ERK信号通路,这可能是其诱导HepG-2细胞的凋亡的机制之一.To study the effect of sulforaphane （SFN） on the protein expression of ERK and p-ERK in human hapetocelluar carcinoma HepG-2 cells and to explore the inducing apoptotic mechanism of SFN on HepG-2 cells. Western Blot assay was employed to detect the expression of ERK and p-ERK proteins. The results showed that after treated by SFN at the dosage of 10, 20, 40 μg/mL for 48 h, the expression of p-ERK proteins was evidently down regulated, meanwhile, there was no remarkable change on the expression of ERK protein. A conclusion can be drawn that SFN can inhibit ERK pathway of HepG-2 cells, which may be one of the mechanism of SFN induced apoptosis of HepG-2 cells.

关 键 词：莱菔硫烷 人肝癌HEPG-2细胞 ERK p—ERK 

分 类 号：R258[医药卫生—中医内科学]