益气活血中药黄芪丹参对急性肺损伤模型大鼠TXB_2、6-Keto-PGF_(1α)的影响  

Effect of supplementing Qi and activating blood circulation herbs astragalus mongholicus and salvia miltiorrhiza on TXB_2 and 6-Keto-PGF_(1α) in plasm of ALI model rat induced by LPS

作　　者：杨明会[1] 张海燕[1] 王文明[2] 刘毅[1] 李绍旦[1] 

机构地区：[1]中国人民解放军总医院中医研究所,北京100853 [2]北京中医药大学

出　　处：《环球中医药》2011年第4期253-255,共3页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(30873344)

摘　　要：目的探讨益气活血中药黄芪丹参对急性肺损伤模型大鼠血栓素B2(thromboxaneB2,TXB2)、6-酮前列腺素F1α(6-keto-prostaglandinF1α,6-Keto-PGF1α)的影响。方法 30只SD雄性大鼠,随机分为正常对照组、模型对照组、中药组各10只,模型对照组和正常对照组每日予以生理盐水2ml灌胃,连续7天,中药组每日予以中药煎液2ml(相当于生药0·5g)灌胃,连续7天。模型对照组、中药组动物采用脂多糖气管滴入法制造急性肺损伤模型,正常对照组气管滴入同等体积生理盐水。采用放射免疫法分别检测各组大鼠血浆TXB2、6-Keto-PGF1α含量的变化。结果中药组大鼠的血浆TXB2含量为(902·35±40·07)pg/ml,模型对照组大鼠的血浆TXB2含量为(1259·09±32·72)pg/ml,中药组低于模型对照组(P<0·01);中药组大鼠的血浆6-Keto-PGF1α含量为(222·58±30·06)pg/ml,模型对照组大鼠的血浆6-Keto-PGF1α含量为(121·64±17·72)pg/ml,中药组高于模型对照组(P<0·01);中药组与正常对照组比较无明显差异(P>0·05)。结论益气活血中药黄芪丹参可能通过维持TXA2/PGI2的相对平衡起到对模型大鼠急性肺损伤的防治作用。Objectives To investigate the effect of supplementing Qi and activating blood circulation herbs astragalus mongholicus（huangqi） and salvia miltiorrhiza（danshen） on TXB2and 6-Keto-PGF1α in plasm of acute lung injury（ALI） model rat induced by lipopolysacchride（LPS）.Methods 30 male sprague dawley rats were randomly divided into normal control group,model control group,herbs group,10 mice in each group.Model control group and normal control group were treated with intragastric administration of 2ml saline daily for one week.Herbs group were treated with intragastric administration herbs liquid（0.25g/ml） 2ml daily for one week.ALI acute lung injury model was made by LPS dripped into tracheal.The content of TXB2and 6-Keto-PGF1αin plasm was tested by radioimmunoassay.Results TXB2in plasma of herbs group was（902.35 ±40.07） pg/ml,while in model control group was（1259.09 ± 32.72）pg/ml,with statistical significance（P0.01）.6-Keto-PGF1αin plasma of herbs group was（222.58±30.06）pg/ml,while in model control group was（121.64±17.72）pg/ml,also with statistical significance（P0.01）.There were no statistical significances between herbs group and normal control group（P0.05）.Conclusion Supplementing qi and activating blood circulation herbs astragalus mongholicus and salvia miltiorrhiza can be used to the prevention and treatment of ALI probably by maintaining the balance of TXA2/PGI2.

关 键 词：黄芪 丹参 急性肺损伤 益气活血 中药 血栓素B2 6-酮前列腺素F1Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]