DWT-PLS在SELDI-TOF MS血清多肽谱解析中的应用  

Application of DWT-PLS Method to SELDI-TOF MS Serum Peptide Profile

作　　者：杨晓丽[1] 侯能邦[1] 李阳[1] 

机构地区：[1]曲靖师范学院化学化工学院,云南曲靖655011

出　　处：《曲靖师范学院学报》2011年第3期31-34,共4页Journal of Qujing Normal University

基　　金：云南省科技计划项目(2009CD095);曲靖师范学院重点项目(2008ZD003)

摘　　要：针对表面增强激光解吸/离子化飞行时间质谱(SELDI-TOF MS)血清多肽谱解析,首先采用离散小波变换(discrete wavelet transform,DWT)对数据进行滤噪、压缩;再采用偏最小二乘(partial leastsquares,PLS)法建立癌症诊断预测模型.考察了DWT对血清多肽谱数据预滤噪、压缩的效率,同时重点研究了小波分解尺度、主成分数对诊断预测准确率的影响.研究结果表明,采用DWT-PLS法解析血清多肽谱简便、高效、预测准确.The SELDI-TOF MS serum peptide profile is dealt through the discrete wavelet transform（DWT） and partial least squares（PLS）.The spectral background and noise are filtered,and the profile was compressed by DWT.Then,the prediction model was constructed by PLS for cancer prognosis.The efficiency of DWT and PLS was studied and the influences of parameters in method were considered.The results indicated that DWT-PLS was convenient,high efficient and accurate.

关 键 词：血清多肽谱 DWT PLS 

分 类 号：O657.6[理学—分析化学]