肉食品中常见4种抗生素残留的高效液相色谱分析  

Simultaneous Determination of Macrolide and Chloramphenicols Antibiotic Residues in Animal Derived Food by HPLC Method

作　　者：纳鹏军[1] 龚波林[1] 

机构地区：[1]宁夏大学化学化工学院,宁夏银川750021

出　　处：《分析测试技术与仪器》2011年第2期65-68,共4页Analysis and Testing Technology and Instruments

基　　金：宁夏自然基金项目(NZ0917)

摘　　要：建立了同时测定肉食品中泰乐菌素、替米考星、氯霉素、氟苯尼考的高效液相色谱方法,样品经乙腈提取,正己烷脱脂,HLB小柱净化后,以C18反相色谱柱为分离柱,甲醇-磷酸二氢钠缓冲溶液（pH 3.0,含体积比为10%的甲醇溶液）为流动相进行梯度洗脱,检测波长λ为275 nm.泰乐菌素、替米考星、氯霉素、氟苯尼考的线性范围是0.1~20.0 mg/L,相关系数分别为0.9987、0.9992、0.9985、0.9970.其平均回收率为75%~87%,相对标准偏差为1.35%~5.41%,泰乐菌素、替米考星、氯霉素、氟苯尼考的检出限分别为20、32、19、16μg/kg.方法满足肉食品中泰乐菌素、替米考星、氯霉素、氟苯尼考的残留量测定.An HPLC method for the simultaneous determination of residues of tylosin（TYL）,tilmicosin（TIL）,chloramphenicol（CAP） and florfenicol（FF） in animal-derived food was developed.The muscle tissue samples were extracted by acetonitrile,then defatted with hexane,and cleaned up on a HLB SPE column.The analytes were separated on a reversed-phase C18 column with the methanol-20 mmol/L NaH2PO4 solution（pH=3.0,containing 10% methanol）,the UV detector was set at λ=275 nm.The standard curves were linear in the range of 0.1～20.0 mg/L for all analytes with correlation coefficients of 0.9970～0.9992.The detection limits for tylosin,tilmicosin,chloramphenicol（CAP）,and florfenicol（FF）,were 20 μg/kg,32 μg/kg,19 μg/kg,16 μg/kg,respectively.The average recoveries of four analytes ranged from 75% to 87%.The relative standard deviations were between 1.35%～5.41%.The method can meet the requirement for the simultaneously determination of tylosin,tilmicosin,chloramphenicol（CAP）,and florfenicol（FF） in beef products.

关 键 词：大环内脂类 氯霉素类 高效液相色谱 

分 类 号：O657.7[理学—分析化学]