2株鸽源新城疫病毒的毒力鉴定和基因型分析  

Phylogenetic Analysis and Virulence Identification of Two Newcastle Disease Virus Isolates from Pigeons

作　　者：高以明[1,2] 张敬友[3] 王水明[2] 柯家法[3] 李超美[2] 朱雪良[2] 张扬[3] 严继宁[2] 黄立业[2] 任晓进[2] 吴艳涛[4] 刘文博[4] 

机构地区：[1]南京农业大学,江苏南京210095 [2]南京出入境检验检疫局,江苏南京210001 [3]江苏出入境检验检疫局,江苏南京210001 [4]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《中国动物检疫》2011年第7期36-39,共4页China Animal Health Inspection

基　　金：江苏检验检疫局科技计划项目(2007KJ03);江苏省高校自然科学基础研究面上项目(07KJB230140);江苏省教育厅指导计划项目(06KJD230211)

摘　　要：2008年从临床鸽病例中分离到2株新城疫病毒毒株,经测定,鸡胚平均死亡时间(MDT)分别为48h和46h,8周龄SPF鸡静脉接种指数(IVPI)分别为2.88和2.91,一日龄雏鸡脑内接种指数(IPCI)分别为1.88和1.89,按照OIE推荐的标准均为强毒株。RT-PCR扩增出融合蛋白(F)基因片段,测序,推导的氨基酸序列中蛋白酶裂解位点附近的氨基酸序列均符合强毒特征序列。根据F蛋白的部分基因序列绘制的系统进化发生树和F基因片段中3种限制性内切酶(RE)位点分布判定这2个毒株均为基因Ⅶc型。Two Newcastle disease virus isolates were got from dead pigeons in 2008.The virulence of the isolates were identified based on the MDT, ICPI and IVPI. The genes of fusion protein were sequenced.The amino acid sequence between 112 to 117 were l12R （K）RQKR Fll7.The restriction enzyme cleavage site mapping of the fusion protein gene between nucleotides 334 and 1682 and by sequence analysis between nucleotides 47 and 420.Both methods revealed the two isolates belong to genotype Vile.The characters of the isolates were as same as other isolates of this genotype from geese and chickens in recent years.It was shown that the virulence and genotype of the Newcastle disease virus from pigeons had changed.

关 键 词：鸽 新城疫病毒 毒力 遗传发生树 基因型 

分 类 号：S852.695.5[农业科学—基础兽医学]