旋覆花素体外抗肿瘤作用研究  

Study on the anti-tumor effect of inulicin in vitro

作　　者：魏海青[1] 李军霞[2] 王永利[2] 

机构地区：[1]石家庄医学高等专科学校,石家庄市050017 [2]河北医科大学药理教研室

出　　处：《河北医药》2011年第13期1938-1940,共3页Hebei Medical Journal

摘　　要：目的探讨旋覆花素对小鼠肝癌H22细胞株、小鼠肉瘤S180细胞株、人肺腺癌A549细胞株、人卵巢癌SK-OV3细胞株及人宫颈癌Hela细胞株的生长抑制作用并将同一浓度对以上5种肿瘤细胞的生长抑制作用进行对比,了解其对以上5种肿瘤细胞的生长抑制作用特点。方法 (1)用MTT比色法测定旋覆花素对肿瘤细胞的生长抑制作用,并计算半数抑制浓度IC50;(2)倒置显微镜下及激光共聚焦显微镜下观察旋覆花素对以上5种肿瘤细胞形态的影响。结果旋覆花素对肿瘤细胞的生长有抑制作用且有一定的选择性,对Hela细胞的抑制作用均较低,旋覆花素对H22、S180、A549及SK-OV3 4种肿瘤细胞的生长抑制作用更明显,倒置显微镜下及激光共聚焦显微镜下可看到明显的形态学变化。结论 旋覆花素具有抗肿瘤作用,并有细胞株选择性。Objective To compare the inhibitory effects of inulicin on the cell growth of mouse hepatoma cell line（H22）,mouse sarcoma cell line（S180）,human lung adenocarcinoma cell line（A549）,human ovarian cancer cell line（SK-OV3） and human cervical cancer cell line（Hela）.Methods MTT method was used to detect the inhibitory effects of inulicin on the cell growth of the 5 cell lines mentioned above,and the IC50 was calculated.The effects of inulicin on morphology of the 5 cell lines were observed by using laser scanning confocal microscope and inverted microscope.Results Inulicin inhibited the cell growth of the 5 cell lines in vitro,but the inhibitory effects of inulicin on S180,SK-OV3,H22 and A549 were more obvious than those on Hela cell,with obvious morphological changes being showed by laser scanning confocal microscope and inverted microscope.Conclusion The inulicin has anti-tumor effect and the effect is selective for different cell lines.

关 键 词：旋覆花素 MTT比色法 抗肿瘤药 植物 

分 类 号：R286.91[医药卫生—中药学]