欧洲水仙鳞片组织培养与快速繁殖  

A study on the scale tissue culture and rapid propagation of Narcissus pseudonarcissus

作　　者：王小敏[1] 李维林[1] 朱洪武[1] 吴文龙[1] 胡淑英[1] 

机构地区：[1]江苏省.中国科学院植物研究所,南京210014

出　　处：《林业科技开发》2011年第4期82-85,共4页China Forestry Science and Technology

基　　金：江苏省农业三项工程项目(编号:SX(2005)077);江苏省科技基础设施建设计划项目(编号:BM2010458)

摘　　要：以欧洲水仙‘Dutch Master’为研究材料,通过对鳞片取材部位、培养光照、激素配比以及组培苗移栽基质的研究发现,欧洲水仙初代培养的较适外植体为内层鳞片下部,合适光照度为1 000 lx。愈伤组织诱导培养基为MS+6-BA 2.0 mg/L+2,4-D 0.3 mg/L+蔗糖30 g/L,小鳞茎的诱导分化培养基为MS+6-BA 2.0 mg/L+NAA 0.2mg/L+蔗糖30 g/L,小鳞茎继代增殖培养基为MS+6-BA 1.5 mg/L+NAA 0.3 mg/L+蔗糖30 g/L,生根培养基为1/2MS+6-BA 0.3 mg/L+NAA 0.5 mg/L+蔗糖20 g/L。炼苗移栽基质配比为V(蛭石)∶V(泥炭土)∶V(园土)=1∶1∶2。Material positions of scale,culture illumination intensity,hormone combination and transplanting medium were studied for tissue culture of Narcissus pseudonarcissus cv.＇Dutch Master＇.The results showed that the suitable explant was the lower part of inner layer scale,the optimal illumination intensity was 1 000 lx,the optimal medium for callus induction was MS＋ 6-BA 2.0 mg/L＋2,4-D 0.3 mg/L＋sucrose 30 g/L,the optimal medium for differentiation was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L＋sucrose 30 g/L,the optimal medium for secondary culture was MS＋6-BA 1.5 mg/L＋NAA 0.3 mg/L＋sucrose 30 g/L,the optimal medium for rooting was 1/2 MS＋6-BA 0.3 mg/L＋NAA 0.5 mg/L＋sucrose 20 g/L,the suitable ratio of transplanting medium was volume（vermiculite）∶volume（peaty soil）∶volume（garden soil）=1∶1∶2.

关 键 词：欧洲水仙 鳞片 组织培养 

分 类 号：S682.21[农业科学—观赏园艺]