红白忍冬的染色体及核型分析  

Chromosome and karyotype analysis of Lonicera japonica var.chinensis

作　　者：武欣[1] 梅树模[2] 郑丽[2] 汪冶[2] 

机构地区：[1]怀化医专红星医院,湖南怀化418000 [2]怀化医专中医药现代化研究中心,湖南怀化418000

出　　处：《中南药学》2011年第6期416-419,共4页Central South Pharmacy

基　　金：2008年度中医药行业科研专项中药类资助项目(No.200807020);湖南省科技厅社会发展支撑计划项目(No.2008SK3034)

摘　　要：目的对红白忍冬[Lonicera japonica Thunb.var.chinensis(Wats.)Bak.]的染色体数目和核型进行研究。方法采用常规制片方法,经显微摄影后对染色体进行分析。结果红白忍冬的体细胞染色体数目2n=18,核型公式为K(2n)=2x=18=2m+12sm(2SAT)+4st(4SAT),染色体相对长度组成为2n=18=4L+2M2+10M1+2S,染色体总长为31.269μm,长臂总长为21.963μm,核型不对称系数(AS.K)为70.24%,属于"3B"型。结论红白忍冬体细胞染色体的数目、核型等清晰准确。Objective To determine chromosome number and karyotype of Lonicera japonica Thunb.var.chinensis（Wats.） Bak.Methods Regular preparation combined with micrograph was used to analyze the chromosome.Results Lonicera japonica var.chinensis normal diploid 2n=18,karyotype formula was K（2n）=2x=18=2m＋12sm（2SAT）＋4st（4SAT）,the relative length was 2n=18=4L＋2M2＋10M1＋2S,the total length of chromosome groups was 31.269 μm,the total length of long arms was 21.963 μm,and the AS.K was 70.24%.Lonicera japonica var.chinensis normal belonged to ＂3B＂type.Conclusion The chromosome number and karyotype morphological characters of Lonicera japonica var.chinensis,etc.are clear.

关 键 词：红白忍冬 染色体 随体 核型分析 金银花 

分 类 号：R282.71[医药卫生—中药学]