反相高效制备液相色谱法制备洋川芎内酯H和I  

Isolation and purification of senkyunolide H and I by preparative reversed-phase high performance liquid chromatography

作　　者：张翠萍[1] 李行诺[1] 陈鸳谊[1] 葛志伟[2] 颜继忠[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032 [2]浙江大学药物信息学研究所,浙江杭州310058

出　　处：《浙江工业大学学报》2011年第4期386-389,共4页Journal of Zhejiang University of Technology

基　　金：国家重大科技专项"重大新药创制"资助项目(2009ZX09313-036)

摘　　要：从川芎中分离制备洋川芎内酯H和洋川芎内酯I对照品.用8倍量80%乙醇回流提取川芎药材,得到川芎浸膏经D-101大孔吸附树脂、硅胶柱色谱及反相高效制备液相色谱分离,获得两个化合物单体.经核磁共振波谱分析,确定其分别为洋川芎内酯H和洋川芎内酯I,两者纯度均高于98%,可作为对照品进行定量分析.该方法操作简单,重现性好,上样量大,能够同时获得大量的高纯度的目标成分,为洋川芎内酯H和洋川芎内酯I单体的制备研究提供了一种新的方法.Senkyunolide H and I were separated and purified from Ligusticum chuanxiong by using 8 times the amount of 80% ethanol to extract Ligusticum chuanxiong Hort.The ethanol extract was subjected to D101 macroporous adsorption resin,silica gel,and preparative reversed-phase HPLC for the isolation of target compounds.Their structures were elucidated as senkyunolide H and I by NMR spectral analysis.And the purity of each compound was above 98%.This method is simple,effective,and reliable for the preparation of senkyunolide H and I simultaneously with good reproducibility and high purity.

关 键 词：反相高效制备液相色谱法 川芎 洋川芎内酯H 洋川芎内酯I 

分 类 号：R284.1[医药卫生—中药学]