香鱼嗜水气单胞菌染病后主要靶器官组织病理学观察  

Histopathological Changes in Main Target Organs of AYU(Plecoglossus altivelis) Infected with Aeromonas hydrophila

作　　者：李明云[1] 张呈念[1,2] 苗亮[1] 王成义[1] 陈炯[1] 史雨红[1] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211 [2]温州科技职业学院,浙江温州325006

出　　处：《宁波大学学报（理工版）》2011年第3期1-4,共4页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：973计划前期研究专项(2008CB117015);长江学者和创新团队发展计划(IRT0734)

摘　　要：从患病香鱼的体腔液中分离获得嗜水气单胞菌病原,用培养稀释的菌液,经腹腔注射人工感染健康香鱼,出现典型出血症后,进行相关的组织病理变化的观察.组织病理显示:脾、肝、肠是细菌侵染重要的靶器官;且脾、肝、肠的变化主要表现为广泛的组织细胞变性、坏死,大量单核细胞、浆细胞等免疫细胞增生.病理超微切片观察显示:在患病香鱼脾脏组织中发现大量长2μm、宽0.5μm左右的细菌,脾脏细胞损伤主要表现为线粒体嵴消失、溶酶体增多,细胞间隙增大,细胞内出现空泡;肝脏主要表现为脂肪变性,溶酶体增多,线粒体嵴断裂、肝糖原颗粒减少;患病香鱼肠道细胞间隙变大,核蛋白体大量或全部脱落,线粒体膜破损,嵴断裂成髓样病变,形成髓样小体.In this paper studies are conducted on the pathological changes of blood and related organizations of Plecoglossus altivelis artificially injected with Aeromonas hydrophila,which was first separated from the coelomic fluidthe of the sicken Plecoglossus altivelis and then diluted followed by being cultivated.Pathological changes of Pleccoglossus altivelis tissues（gill,liver,spleen,kidney,heart intestine） were observed,finding that the liver,spleen,intestine are the major organelles which are easily infected.Histopathological changes of liver,spleen,intestine are characterized by extensive cellular degeneration,necrosis,and large number of immune cells such as monocytes and plasma cells proliferation.The findings in ultra-structure are as follows：a kind of bacterium whose length was about 2 μm and breadth was 0.5 μm is identified in spleen.The changes of spleen are characterized by the disappearance of mitochondria ridge,the increase number of lysosome,and the enlargement the intercellular gaps.The pathological changes are also significant in liver and characterized by a series of indicators such as the denaturation of fat,the increase of lysosomes,the rupture of mitochondria ridge,and the decrease of glycogen.And results arising from the pathological changes can be described as folloews：the intestinal cell gap of the infected AYU becomes larger,and the nuclein of it mostly or absolutely sheds;the mitochondrial membrane is damaged;the marrow is broken into the medullary and formed medullary corpuscles.

关 键 词：嗜水气单胞菌 香鱼 组织病理 

分 类 号：S943[农业科学—水产养殖]