葡萄糖和胰岛素对3T3-L1脂肪细胞内脏脂肪素mRNA表达的影响  

Effects of glucose and insulin on the expressions of visfatin mRNA in 3T3-L1 adipocytes

作　　者：李鹏杰[1] 李强[1] 潘红艳[1] 郭琳[1] 于萍[1] 张巾超[1] 

机构地区：[1]哈尔滨医科大学附属第二医院内分泌代谢病科,哈尔滨150086

出　　处：《中国糖尿病杂志》2011年第7期542-544,共3页Chinese Journal of Diabetes

基　　金：教育部高等学校博士学科点科研基金(200802260007);黑龙江省教育厅高等学校骨干教师创新计划(1054C3024)

摘　　要：目的研究不同浓度葡萄糖和胰岛素对3T3-L1脂肪细胞中内脏脂肪素(Visfatin)mRNA表达的影响。方法通过real-time RT-PCR方法检测不同浓度葡萄糖和胰岛素培养下3T3-L1脂肪细胞Visfatin mRNA的表达。结果葡萄糖增加了3T3-L1脂肪细胞Visfatin mRNA的表达;胰岛素降低其表达。结论葡萄糖和胰岛素对3T3-L1脂肪细胞中Visfatin mRNA的表达有调控作用。Objective To investigate the effects of glucose and insulin in different concentrations on the expressions of visfatin mRNA in 3T3-L1 adipocytes. Methods 3T3-L1 adipocytes were treated with different concentration of glucose and insulin. The expressions of visfatin mRNA were measured by quantitative real-time RT-PCR with 36B4 as internal control. Results Glucose increased the expression of visfatin mRNA, hut insulin downregulated visfatin mRNA expressions in 3T3-L1 adipocytes. Conclusions Glucose and insulin regulate the expression of visfatin mRNA in 3T3-L1 adipocytes.

关 键 词：内脏脂肪素 葡萄糖 胰岛素 3T3-L1脂肪细胞 

分 类 号：R587.1[医药卫生—内分泌]