2-型猪链球菌保护性抗原RfeA的B细胞表位预测  

Prediction of B cell epitope for protective antigen RfeA against Streptococcus suis serotype 2

作　　者：刘丽娜[1,2] 潘渠[2] 朱军民[2] 丛延广[2] 刘红美[1] 李程 

机构地区：[1]贵阳医学院基础医学院医学生物技术教研室,贵州贵阳550004 [2]第三军医大学基础医学院微生物学教研室,重庆400038 [3]重庆市永荣矿务局总医院病理科,重庆402460

出　　处：《成都医学院学报》2011年第2期133-135,共3页Journal of Chengdu Medical College

基　　金：贵州省科技厅社发项目(黔科合[2010]3144);贵阳医学院博士启动基金(K2010-32)

摘　　要：以2-型猪链球菌（SS2）保护性抗原RfeA推定的氨基酸序列为基础,采用Kyte-Doolittle法分析蛋白的亲水性,Emini法预测蛋白的表面可能性,以及Jameson-Wolf法预测蛋白的抗原指数;辅以Garnier-Robson法、Chou-Fasman法和Karplus-Schulz法对蛋白二级结构中柔性区域的分析,预测SS2保护性抗原RfeA的B细胞表位。结果表明,推测RfeA的B细胞表位位于RfeA蛋白N-端第21~27、53-60、104~117、140~160区域。用多参数预测SS2保护性抗原RfeA的B细胞表位,为实验研究SS2的多表位疫苗奠定基础。The secondary structure flexible region of protective antigen RfeA against Streptococcus suis serotype 2（S.suis 2）was analyzed by the Garnier-Robson＇s method,the Chou-Fasman＇s method and the Karplus-Schulz＇s method based on their putative amino sequence.Hydrophilicity plot,surface probability plot and antigenicity index were obtained by the methods of Kyte-Doolittle,Emini and Jameson-Wolf,respectively.B cell epitopes for RfeA were predicted based on these results The predicted B cell epitopes for RfeA were located at the N-terminal No.21~27,53-60,104~117,140~160.Taken together,prediction of the B cell epitopes for protective antigen RfeA against S.suis 2 based on multiple parameters would lay foundation for experimental research of multi-epitope vaccine against S.suis 2.

关 键 词：2-型猪链球菌 保护性抗原 B细胞表位 

分 类 号：S852.61[农业科学—基础兽医学]