知母总皂苷对慢性温和应激小鼠的保护作用及机制研究  

Protective Effect of Total Timosaponin on Chronic Mildly Stressed Mice and Its Mechanism

作　　者：任利翔[1] 罗轶凡[1] 高威[1] 白艳梅[1] 宋少江[2] 吴英良[2] 

机构地区：[1]沈阳中海生物技术开发有限公司,沈阳110016 [2]沈阳药科大学,沈阳110016

出　　处：《中药新药与临床药理》2011年第4期414-417,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的探讨知母总皂苷(total timosaponin,TT)对慢性温和应激模型小鼠的保护作用并分析其可能的作用机制。方法建立小鼠慢性温和应激模型,测量小鼠对蔗糖水的摄取量,采用避暗实验考察小鼠学习记忆能力,放射免疫法测定小鼠血浆促肾上腺皮质激素(ACTH)和皮质醇含量,酶联免疫法测定海马脑源性神经生长因子(BDNF)含量。结果 TT(25、50 mg.kg-1,qd×21 d)可显著增加慢性温和应激模型动物的蔗糖水消耗量,延长动物逃避潜伏时间、减少逃避错误次数。生化结果显示,TT能够降低血浆ACTH和皮质醇的含量,提高海马组织内BDNF含量。结论 TT能够改善慢性温和应激模型小鼠的抑郁状态和学习记忆能力,其机制与抑制下丘脑-垂体-肾上腺轴功能亢进以及提高模型动物海马BDNF含量有关。Objective To investigate the protective effect of total timosaponin （TT） on chronic mildly stressed （CMS） mice and to explore its possible therapeutic mechanism. Methods The sucrose solution consumption test was used to observe the antidepressant effect of TT, and step-through test was used for the observation of learning and memory of CMS mice. Plasma levels of adrenocorticotropic hormone （ATCH） and cortisol were detected with radioimmunoassay, and brain-derived neurotrophic factor（BDNF） in the hippocampus was examined with enzyme-linked immunosorbent assay. Results Treatment with TT（25 and 50 mg·kg-1） markedly enhanced the consumption of sucrose solution in CMS models. Administration of TT（50 mg·kg-1） significantly increased escaping latency and decreased the number of errors. Biochemical studies showed that the increase of ATCH and cortisol, and the decrease of BDNF in CMS mice were significantly ameliorated by TT. Conclusion TT could improve the depression state and promote learning and memory impairment in CMS mice. The mechanism may be related with the suppression of the hyperfunction of hypothalamic-pituitary-adrenal axis and with the increase of BDNF level in the hippocampus.

关 键 词：知母总皂苷 慢性温和应激 HPA轴 学习记忆 小鼠 

分 类 号：R285.5[医药卫生—中药学]