清开灵颗粒对AD模型小鼠学习记忆能力的影响  

Effect of Qingkailing Granule on Learning and Memory in Scopolamine-induced Alzheimer's Disease Modle Mice

作　　者：王永丽[1,2] 韩璇[3] 问慧娟[3] 李玉巧[3] 周雅静[3] 魏芳[4] 

机构地区：[1]河北大学临床医学院 [2]河北大学附属医院药剂科,河北保定071000 [3]河北大学基础医学院,河北保定071000 [4]河北大学公共卫生学院,河北保定071000

出　　处：《中药新药与临床药理》2011年第4期421-423,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：河北省科学技术研究与发展计划项目(10276105D-89)

摘　　要：目的研究清开灵颗粒对AD模型小鼠学习记忆的影响。方法选用东莨菪碱造模的雄性KM小鼠,用Morris水迷宫测试行为学指标,采用黄嘌呤氧化酶比色法测定超氧化物歧化酶(SOD)活性、硫代巴比妥酸法测定脂质过氧化产物丙二醛(MDA)含量、三硝基苯比色法测定脑内胆碱酯酶(AchE)活性。结果清开灵颗粒能明显缩短模型小鼠游泳时间及游泳路程;显著提高脑组织SOD活性,降低MDA含量;明显降低脑组织AchE活性。结论清开灵颗粒能改善造模小鼠的学习记忆功能,其机制可能与提高SOD活性、降低脑组织脂质过氧化物的含量、抑制AchE活性有关。Objective To study the effect of Qingkailing granule on learning and memory ability in Alzheimer＇s disease model mice. Methods Male KM mice were used for the experiment, and Alzheimer＇s disease model was induced by scopolamine. Morris water maze was used to observe the learning and memory ability of model mice. Xanthine oxidase colorimetry was used for the examination of superoxide dismutase （SOD） activity, thiobarbituric acid colorimetry for malondialdehyde content, and trinitrobenzene colorimetry for acetylcholinesterase（AchE）activity. Results Qingkailing granule could significantly shorten swimming time and swimming distance in Morris water maze, increase the activity of SOD, and decrease MDA content and AchE activity in the brain tissue of AD model mice. Conclusion Qingkailing granule could improve the ability of learning and memory of model mice, and its mechanism might involve in increasing SOD activity, reducing the level of peroxidation and inhibiting AchE activity in brain tissue.

关 键 词：清开灵颗粒 学习记忆 抗氧化 胆碱酯酶 小鼠 

分 类 号：R285.5[医药卫生—中药学]