巴豆非脂肪油部分HPLC指纹图谱研究  

Study on HPLC Fingerprints of Non-fat-oil Fraction in Fructus Crotonis

作　　者：黄孟秋[1] 曾宝[1,2] 李生梅[1] 唐君苹[1] 袁捷[1,2] 赖小平[1,2] 

机构地区：[1]广州中医药大学,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中药新药与临床药理》2011年第4期444-447,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技部重大新药创制专项(2009ZX09103-388)

摘　　要：目的建立巴豆非脂肪油部分的高效液相色谱指纹图谱,为其质量评价提供理论依据。方法采用RP-HPLC法,测定了10批巴豆样品。色谱条件:Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相:A相为0.2%醋酸,0.3%三乙胺溶液,B相为乙腈,梯度洗脱,体积流量:0.8 mL/min,柱温:30℃,检测波长:247 nm。结果在色谱指纹图谱中,确立了其中12个共有峰,建立了巴豆非脂肪油部分的共有模式,10批样品指纹图谱相似度在0.982～0.995之间。结论该方法简便、准确、重现性好,为评价巴豆药材的质量提供了依据。Objective To establish the HPLC fingerprint of non-fat-oil fraction in Fructus Crotonis, and to offer evidence for quality evaluation of Fructus Crotonis. Methods We measured ten batches of Fructus Crotonis By using RP-HPLC. The Separation Condition was as follows： Agilent Eclipse XDB-C18 chromatographic column（250 mm × 4.6 mm, 5 μm） , with 0.2 % acetic acid and 0.3 % triethylamlne solution as mobile phase A, and with acetonitrile as mobile phase B, gradient mode, the flow rate being 0.8 mL/min, column temperature at 30℃, and detection wavelength at 247 nm. Results The common modes for the HPLC fingerprints of non-fat-oil fraction in Fructus Crotonis were set up by ensuring twelve common peaks in the chromatograms. The similarity degrees in the ten batches were in the range of 0.982～0.995. Conclusion The established method is simple and accurate, and have a good repeatability, which will supply evidence for the quality evaluation of medicinal material of Fructus Crotonis .

关 键 词：巴豆 巴豆苷 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]