青黛药材中的靛蓝和靛玉红含量的同时测定  

Simultaneous Determination of Indigo and Indirubin in Medicinal Material of Indigo Naturalis

作　　者：谢友良[1,2] 何百寅[1] 李远彬[1] 叶雪兰 刘常青[1] 陈建南[1,2] 赖小平[1,2] 

机构地区：[1]广州中医药大学新药研究开发中心,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,东莞523808 [3]广州潘高寿药业股份有限公司,广州511490

出　　处：《中药新药与临床药理》2011年第4期452-455,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：东莞市科技计划重点项目(2008B090200004);新型巴布剂的研发及其产业化应用

摘　　要：目的以HPLC法同时测定青黛中有效成分靛蓝和靛玉红的含量。对中国药典2010年版中青黛含量测定方法的不足之处进行改进。方法用适量DMF超声30 min提取青黛药材中的靛蓝和靛玉红。液相条件如下:色谱柱:Kromasil C18(250 mm×4.6 mm,5μm);流速1 mL/min;流动相:乙腈∶水(70∶30);柱温:30℃;检测波长:290 nm。此法在不影响分析结果的条件下可大大缩短分析时间。结果靛蓝和靛玉红进样量在29.90 ng^298.90 ng和6.71 ng^67.06 ng间与峰面积的线性关系良好(r>0.999,n=6)。靛蓝平均加样回收率:101.05%(RSD=1.81%);靛玉红平均加样回收率:99.78%(RSD=2.52%)。结论本法合理、便捷;能同时测定青黛中靛蓝和靛玉红含量,符合现行药典简便、快速、准确的要求。Objective To establish a HPLC method for simultaneous determination of indigo and indirubin in medicihal material of Indigo Naturalis, and to improve the quality standard of Indigo Naturalis in Chinese Pharmacopeia of 2010 edition, volume I. Methods We use suitable amount of DMF to extract indigo and indirubin from Indigo Naturalis simultaneously by uhrasonography for 30 rain. HPLC condition were as follows： Kromasil C18 （250 mm×4.6 mm, 5μm）, mobile phase of acetonitrile ： water（70 ： 30）, column oven temperature at 30℃ , and detection wavelength at 290 nm. The above HPLC conditions had no effect on the analysis result but could greatly shorten analysis time. Results The linear correlation of the indigo was good in the range of 29.90 ng-298.90 ng and the recovery was 101.05 % （RSD=I.81%）. The Linear correlation of the indirubin was good in the range of 6.71 ng-67.06 ng and the recovery was 99.78 %（RSD=2.52 %）. Conclusion This method is simple, rapid and effective for simultaneous determination of indigo and indirubin in Indigo Naturalis, which meets the requirement of current pharmacopoeia.

关 键 词：青黛 靛蓝 靛玉红 HPLC法 

分 类 号：R284.1[医药卫生—中药学]