中国海南黎族人群TAP1基因多态性与结核病的关联性研究  

Association between TAP1 gene polymorphism and tuberculosis in Li natinoality in Hainan China

作　　者：吉丽敏[1] 王丹妹[1] 莫燕娜[1] 何佟[1] 林峰[2] 林容[3] 林堂昌[2] 

机构地区：[1]海南医学院机能学实验室,海口市571101 [2]海南省疾病预防控制中心,570203 [3]海南医学院,海口市570105

出　　处：《实用医学杂志》2011年第14期2515-2518,共4页The Journal of Practical Medicine

基　　金：海南省自然科学基金资助项目(编号:30714)

摘　　要：目的:研究海南黎族人群抗原处理相关转运体基因1(TAP1)多态性与肺结核病(PTB)易感性的关系。方法:应用PCR-RFLP技术对205例海南黎族肺结患者及217例海南黎族健康人群TAP1-1/TAP1-2(即Codon333/637)多态性进行检测。并对其易感性进行分析。结果:(1)海南黎族人群TAP1-1/TAP1-2基因多态性位点具有多态性(A→G);(2)TAP1-1Codon333多态性位点纯合型Val/VaL的基因频率病例组为4.88%,低于对照组5.33%,而病例组的杂合型Ile/Val频率为44.39%,明显高于对照组26.73%,在两组人群中,野生型Ile/Ile与杂合型比较,P=0.008,OR=1.92(95%CI:1.19～3.12),差异有意义,而与纯合型比较,无差异(P>0.05);(3)TAP1-2Codon637多态性位点纯合型Gly/Gly、杂合型Asp/Gly的基因频率在两组人群中的差异有高度显著性(P<0.01),其中野生型与杂合型比较OR=2.87(95%CI=1.75～4.71),与纯合型比较,OR=3.94,(95%CI=1.82～8.53)。结论:TAP1基因Codon637位点多态性可能是海南黎族人群肺结核的易感因素。Objective To study the correlation between the transporter associated with antigen processing genel （TAP1） gene polymorphism and the susceptibility to tuberculosis in Li nationality from Hainan province in China. Methods The TAPI-I/TAP1-2 gene polymorphism in 205 patients （case group） with tuberculosis and 217 cases of healthy controls （control group） were detected by restriction fragment length polymorphism （RFLP）, and their susceptibility to pulmonary tuberculosis were analyzed. All the patients and healthy controls were Li nationality from Hainan province in China. Results There were gene polymorphism （A→G） of TAPI-1/TAP1-2 in Li nationality from Hainan province. The distributions of Val/VaL in the polymorhic site of TAPl-lCodon333 were 4.88% and 5.33% in case group and control group, respectively; those of Ile/Val were 44.39% and 26.73%, respectively; there was significant difference between the distributions of Ile/Ile and Ile/Val in both groups [P = 0.008,OR = 1.92 （95% CI： 1.19 - 3.12）1. There were significant differences in the distributions of Gly/Gly and Asp/Gly in TAP1-2Codon637 between the two groups （P 〈 0.01）. The distribution of Asp/Asp was different from those of Asp/Gly [P〈 0.01,OR = 2.87 （95% CI= 1.75 - 4.71）] and Gly/Gly [OR = 3.94 （95% CI = 1.82 8.53）]. Conclusion Polymorphisms of Codon637 locus in TAPI gene may be the predisposing factors fbr tuberculosis in Li nationality from Hainan province in China.

关 键 词：结核 肺 抗原处理相关转运体 黎族 

分 类 号：R52[医药卫生—内科学]