丹参多酚酸盐对急性敌敌畏中毒大鼠的心肌保护作用  

Protective Effect of Salvianolate on Myocardial Injury in Rats with Acute Dichlorvos Poisoning

作　　者：汤罗嘉[1] 姚晨玲[1] 黄培志[1] 汤彦 钱欣[3] 

机构地区：[1]复旦大学附属中山医院急诊科,上海200032 [2]上海市青浦区中心医院,上海201700 [3]福建省立医院,福建福州350001

出　　处：《中国临床医学》2011年第3期279-280,301,共3页Chinese Journal of Clinical Medicine

基　　金：国家科技支撑计划专题基金(编号:2006BAI06B01)

摘　　要：目的:观察丹参多酚酸盐对急性有机磷中毒(acute organophosphorus pesticides poisoning,AOPP)心肌的保护作用。方法:Sprague Dawley(SD)大鼠24只,随机分成3组,均予以气管切开接呼吸机辅助通气,中毒组予以腹腔注射敌敌畏(dichlor-vos)60mg/kg;治疗组在腹腔注射敌敌畏的同时予以腹腔注射丹参多酚酸盐10mg/kg,对照组仅注入同等量的0.9%氯化钠液。1h后留取血样测血清丙二醛(MDA)、超氧化物岐化酶(SOD)、心肌钙蛋白T(cardiac troponin T,CTnT)水平。取左心室心肌组织用原位缺口末端标记(TUNEL)法染色,检测凋亡指数(apoptotic index,AI)。结果:与中毒组比较,治疗组血清CT-nT、MDA值显著降低,SOD值显著升高(P<0.01)。心肌细胞凋亡指数显著下降(P<0.01)。结论:AOPP时有显著的氧化应激反应和细胞凋亡发生。应用丹参多酚酸盐可减轻氧化应激反应,降低血清CTnT水平,减少心肌细胞凋亡,有保护心肌作用。Objective：To investigate the protective effect of salvianolate on the myocardial injury in rats with acute organophosphorus pesticides poisoning.Methods：A total of 24 male Spragne-Dawley（SD） rats were randomized into the control group,poisoning group and treated group（n=8 each）.Three groups were all supported with breathing machine connected with the tube from incision of trachea.The poisoning model was established by peritoneal injection of dichlorvos（60mg/kg 2LD50）.The treated group was peritoneal injected salvianolat（10mg/kg） while rats were poisoned.Superoxide dismutase（SOD）,malondialdehyde（MDA）and cardiac troponin T（CTnt） level in blood serum were determined after an hour.Myocardial apoptosis was detected by TdT-mediated dUTP biotin nick end labeling（TUNEL）method.Apoptosis index was calculated under an optical microscope.Results：Compared with the poisoning group,the level of CTnt,MDA and AI in the treated group were significant decreased while SOD was increased.（P0.01）.Conclusions：It shows that reducing oxidative stress by salvianolate might be one of the protctive cardial mechanisms.It decreases the level of CTnt and myocardial poptosis.

关 键 词：敌敌畏 丹参多酚酸盐 凋亡 

分 类 号：R595.4[医药卫生—内科学]