高效液相色谱法测定银芩胶囊中黄芩苷的含量  

Determination of Baicalin in Yinqin Jiaonang by HPLC

作　　者：颜滢 李卓[2] 

机构地区：[1]湖南省衡阳市中心医院,421001 [2]湖南省南华大学附一医院,421001

出　　处：《首都医药》2011年第14期54-55,共2页Capital Medicine

摘　　要：目的探讨银芩胶囊中黄芩苷的含量及测定方法。方法采用高效液相色谱法,色谱柱C18柱,流动相为甲醇-磷酸-四氢呋喃(45:55:6)(磷酸为pH=2.7的磷酸水溶液),流速为1.0ml/min,检测波长280nm。结果黄芩苷进样量在3.7～29.6μg/ml范围内与峰面积积分值呈良好的线性关系,标准曲线Y=57417x-31303,γ=0.9998,平均回收率为98.89%,RSD=0.62%(n=6)。结论本方法简便、准确、重现性好,可用于该制剂的质量控制。Objective To determine the baicalin in Yinqin Jiaonang.Methods HPLC method has been developed.The moble phase was CH3OH-H2O-Tetrahydrofuran （THF） （45：55：6） （H3PO4,pH=2.7）.The flow rate was 1.0ml/min.The detective wavelength was 280nm.Results The linear concentration range of baicalin was 3.7～29.6μg/ml,the calibration curve wasY=57417x-31303,γ=0.9998,the average recovery standard deviation was 98.89%,and RSD was 0.62% （n=6）.Conclusion This method is simple,accurate and reproducible,and it can be used for the quality control of baicalin.

关 键 词：高效液相色谱法 银芩胶囊 黄芩苷 含量测定 

分 类 号：R927.2[医药卫生—药学]