HIF-1α VEGF和FGF在胰腺癌中的表达及临床意义  

作　　者：杨士勇[1] 时开网[1] 姚平[1] 

机构地区：[1]南京医科大学附属南京市第一医院,210006

出　　处：《浙江临床医学》2011年第7期721-725,共5页Zhejiang Clinical Medical Journal

基　　金：南京医科大学重点课题资助项目（08NMUZ037）

摘　　要：目的研究缺氧诱导因子1alpha（Hypoxia—inducible factor-1alpha，HIF-lα）、血管内皮生长因子（vascular endothelial growth factor，VEGF）和成纤维细胞生长因子（fibroblast growth factor，FGF）在胰腺癌组织中的相关性表达及其与临床病理特征之间的关系。方法分别应用免疫印迹Western blot法和实时荧光定量检测22例胰腺癌及相应正常胰腺组织中HIF-1α、VEGF和FGF的表达，分析其间的相关性以及与肿瘤临床病理特征的关系。结果HIF-lα,VEGF和FGF在胰腺癌组织中的表达水平显著高于胰腺癌周组织（P〈0．01），三者之间的表达具有显著相关性（P〈0．01）。HIF-1α的表达与胰腺癌的TNM分期、肿瘤大小和淋巴结转移有关，VEGF和FGF的表达与胰腺癌的肿瘤大小和淋巴结转移有关。结论HIF-lα、VEGF和FGF在胰腺癌的发生、发展中起着重要作用，联合检测三种细胞因子对预测术后胰腺癌患者的预后具有一定的作用。Objective To investigate the related expressions of hypoxia -inducible factor- 1 alpha （HIF- l α）, vascularendothelial growth factor（VEGF） and fibroblast growth factor（FGF） as well as the relationships between them and clinical pathology characters in human pancreatic carcinoma. Methods The mRNA and protein expression of HIF - 1α, VEGF and FGF in 22 cases of resected specimen of pancreatic carcinoma and their surrounding pancreatic tissues were detected by western blot method and realtime fluorescence quantitative polymerase chain reaction technique, respectively. Their relationships to the clinicopathologic characteristics of human pancreatic carcinoma were analyzed. Results Higher expressions of HIF - 1α, VEGF and FGF were detected in pancreatic carcinoma compared with those in normal pancreatic tissues （P 〈 0. 01 ）. Furthermore, significant correlations were observed between HIF - lα and VEGF or FGF（P 〈0. 01 ）. The expressions of HIF -lα were closely related to TNM staging, tumor size of pancreatic carcinoma and the lymphnode metastasis. The expressions of VEGF and FGF were closely related to tumor size of pancreatic carcinoma and the lymphnode metastasis. Conclusions HIF - lα, VEGF and FGF are overexpressed in pancreatic carcinoma and play an important role in the origin and development of pancreatic carcinoma, The combined detection of HIF - 1α, VEGF and FGF is of certain significance in predicting the prognosis of patients with undergoing surgery.

关 键 词：胰腺癌 缺氧诱导因子-1Α 血管内皮生长因子 成纤维细胞生长因子 

分 类 号：R651.2[医药卫生—外科学]