FgBβ1689T/G、I6I/D、345C/T和HinfIA/C基因多态性与血浆Fg功能表达的相关性研究  

The relationship between the polymorphisms of FgBβ 1689T/G,I6I/D,345C/T,and Hinf IA/C and functional expression of Fibrinogen

作　　者：徐建辉[1] 元小冬[1] 张楠楠[1] 李宏芬[1] 邓红亮[1] 

机构地区：[1]河北联合大学附属开滦医院神经内科,唐山063000

出　　处：《中国免疫学杂志》2011年第7期634-638,共5页Chinese Journal of Immunology

摘　　要：目的:分析血浆纤维蛋白原(Fg)Bβ1689T/G、I6I/D、345C/T和Hinf IA/C多态性位点对Fg浓度和分子聚合活性等功能表达指标的影响。方法:采用整群抽样的方法选取开滦集团职工1 021人,均留取清晨空腹静脉血测定血糖、尿酸等生化指标;分别应用PCR-RFLP和基因测序技术确定四位点的基因型;采用微机辅助血浆Fg功能自动监测系统测定血浆Fg浓度和Fg单体聚合反应速率(FMPV)、最大光密度(Amax)、FMPV/Amax等反映Fg分子聚合功能参数。结果:Fg浓度、FMPV、Amax及FMPV/Amax在Bβ1689、I6、345、Hinf I各基因型组间比较均无显著性差异(P>0.05);FMPV/Amax指标在两性之间的差异有统计学意义(P<0.05);在无高血压病的人群中BβHinf I的AA组FMPV/Amax高于CC+AC组(P<0.05),在无脑梗死的人群中Bβ1689的GG+TG组的FMPV/Amax高于TT组(P<0.05)。结论:Bβ1689位点在特定条件下具有参与和影响调解Fg分子聚合活性表达的作用;BβHinf IA/C发生变异时可以使血浆Fg分子活性降低。Objective：To analyze the relationship be tween the polymorphisms of Bβ-gene 345C/T,I6I/D,1689T/G and Hinf IA/C and functional expression of plasma fibrinogen concentration and molecular reactivity for studying their regulation function to Fg functional expression in order to provide the genetic basis for prevention and diagnosis of thrombotic diseases.Methods：1 021 subjects from Tangshan Kailuan Group were enrolled with study method of cluster sampling;Bβ345C/T,I6I/D,1689T/G and HinfIA/C polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and Sequencing;Fg concentration,fibrin monomer polymerized velocity（FMPV）,absorbance maximum（Amax）,FMPV/Amax and biochemistry indicators including glucose and UA were measured.Results：No difference of Fg concentration,FMPV,Amax and FMPV/Amax in Bβ345C/T,I6I/D,1689T/G and HinfIA/C genotypes was found（P〉0.05）,but difference of FMPV on gender（P〈0.05）;In non-hypertension,FMPV/Amax of AA genotype of Hinf I was higher than that of CC＋AC（P〈0.05）,and in non-Cerebral infarction,FMPV/Amax of GG＋TG genotype of 1689 was higher than that of TT（P〈0.05）.Conclusion：Fg1689 in special condition can take part in and regulate functional expression of Fg molecular reactivity;Variation of Hinf IA/C may decrease Fg molecular reactivity low.

关 键 词：纤维蛋白原 Bβ链 基因多态性 分子聚合功能 

分 类 号：Q592.1[生物学—生物化学]