IL-18与HSV-2gD模拟抗原表位DNA疫苗的免疫效果观察  

The immune responses of the DNA vaccine of IL-18 and HSV-2gD

作　　者：焦凤萍[1] 刘莉[1,2] 于爱莲[1] 王玉[1] 于广福[1] 

机构地区：[1]泰山医学院病原学教研室,泰安271000 [2]聊城市疾病预防控制中心,聊城252000

出　　处：《中国免疫学杂志》2011年第7期654-656,共3页Chinese Journal of Immunology

基　　金：山东省卫生厅资助课题(2007HZ037);山东省自然科学基金(ZR2009CQ003)资助项目

摘　　要：目的:研究串联重组核酸疫苗pcDNA3.1-IL-18-HSVP6和pcDNA3.1-IL-18-HSVNP6对机体的免疫效果。NP6为与HSV-2gD模拟抗原P6最相似的天然抗原表位序列(Accession Number:E00394)。方法:将构建的真核表达质粒pcDNA3.1-IL-18、pcDNA3.1-IL-18-HSVP6和pcDNA3.1-IL-18-HSVNP6肌内注射免疫接种BALB/c小鼠3次,每次间隔1周。末次免疫后1周眼眶静脉采血,ELISA法检测小鼠血清特异性抗体滴度、IFN-γ及IL-18含量;末次免疫后一月,处死小鼠,无菌分离脾脏,MTT法测定脾淋巴细胞增殖率。结果:重组核酸疫苗pcDNA3.1-IL-18-HSVP6免疫小鼠后可刺激血清特异性抗体的产生,诱导分泌较高水平的IFN-γ和IL-18。可促进小鼠脾淋巴细胞的增殖。结论:重组核酸疫苗pcDNA3.1-IL-18-HSVP6能诱导较强的细胞免疫和体液免疫,从而为构建更加有效的HSV-2DNA疫苗奠定了良好的基础。Objective：To study the immune responses of pcDNA3.1-IL-18-HSV P6 and pcDNA3.1-IL-18HSV NP6 genetic vaccine.NP6 is the similar sequence of natural HSV-2 gD from gene bank.Methods：The recombinant plasmid pcDNA3.1-IL-18、pcDNA3.1-IL-18-HSV P6 and pcDNA3.1-IL-18-HSV NP6 were used to inoculate BALB/c mice by intramuscular injection for three times,once a week.One week after the last vaccination,the levels of specific IgG antibody,IFN-γ and IL-18 were detected by ELISA;One month after the last vaccination,spleen cells of vaccinated mice were separated and proliferation of spleen lymphocytes was detected by MTT.Results：After inoculated by pcDNA3.1-IL-18-HSV P6 vaccine,the mice could produce higher level of splenocytes proliferation and secrect IFN-γand IL-18 and specific antibody.Conclusion：the recombinant clone pcDNA3.1-IL18-P6 can effectively induce humoral and cellular immune responses,which offered the basic requirements for constructing new type of DNA vaccine.

关 键 词：DNA疫苗 IL-18 单纯疱疹病毒Ⅱ型 模拟抗原表位 

分 类 号：R392[医药卫生—免疫学]