连续流Biohydrogenbacterium R3 sp.nov.菌株糖蜜废水发酵产氢能力分析  

Continuous Fermentative Hydrogen Production from Molasses with Biohydrogenbacterium R3 sp.nov. in Continuous Stirred-Tank Reactor

作　　者：白羽[1] 蔡体久[1] 韩伟[1] 李永峰[1] 刘海亮[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2011年第8期94-95,105,共3页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(50908062;30870420)

摘　　要：以从连续流搅拌槽式反应器(CSTR)内的活性污泥中分离出的高效厌氧产氢菌BiohydrogenbacteriumR3 sp.nov.为发酵产氢微生物,糖蜜废水为发酵底物,试验分析了连续流发酵法R3菌株的产氢效能。结果表明:在温度36℃、水力停留时间6 h、进水COD在2 600～4 440 mg/L范围内变化,CSTR反应器可实现连续稳定产氢,并且分别得到最大产气量和产氢量为6.08 L和3 L;进水底物质量浓度的变化,对系统产氢效能的影响十分明显。进水pH值的降低影响系统的COD去除效率,但对出水pH值却无明显影响,可见,R3菌株纯培养CSTR是一个相对稳定的发酵制氢系统,系统氧化还原电位稳定在-445～-420 mV。An experiment was conducted to study the performance of hydrogen production with Biohydrogenbacterium R3 sp. nov. isolated from activated sludge from molasses. The reactor was operated at a temperature of 36 degrees C with a hydraulic residenee time of 6 h in continuous flow mode. Experimental data showed the feasibility of continuous hydrogen production with Chemical Oxygen Demand （COD） ranging from 2 600 to 4 440 m/L, and the maximum biogas and hydrogen yield of 6.08 L and 3 L were obtained, respectively. The performance of hydrogen production was obviously affected by influent substrate concentration. The variation of iniluent pH value had an impact on the efficiency of COD removal but no influence on effluent pH value, which indicated that the continuous stirred-tank reactor with R3 was a relative hydrogenproducing system. The oxidation-reduction potential stabilized at -445 ～ -420 mV.

关 键 词：发酵制氢 R3菌株 底物质量浓度 PH值 

分 类 号：Q936[生物学—微生物学] TK6[动力工程及工程热物理—生物能]